湖北省灰葡萄孢抗药性测定及抗药分子机制研究  

Monitoring Fungicide Resistance of Botrytis cinerea from Hubei Province and Molecular Mechanisms of Resistance

作　　者：李若晨[1] 张静[1] 李国庆[1] 

机构地区：[1]华中农业大学国家微生物重点实验室/湖北省作物病害监测与安全控制重点实验室,武汉430070

出　　处：《湖北农业科学》2014年第12期2800-2805,共6页Hubei Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(201303025)

摘　　要：评价湖北省灰葡萄孢（Botrytis cinerea）群体抗药性水平，为防治灰霉病提供理论依据，评估了源于湖北省的96个灰葡萄孢菌株对4种杀菌剂的抗性。结果表明，大部分菌株对多菌灵、农利灵和菌核净敏感，抗性菌株出现的频率分别为5%、2%和3%；所有供试菌株均对环酰菌胺敏感，EC50为0.004~0.200μg/mL；对Bc-hc h基因序列扩增和酶解谱带分析结果进一步证实了供试菌株对环酰菌胺敏感。抗性菌株抗药相关基因β-微管蛋白和组氨酸激酶基因的氨基酸序列比对结果显示，部分位点的突变是抗药性形成的可能机制之一，另外也发现了新的突变位点。Resistance of 96 B.cinerea strains from Hubei Province to four fungicides were studied. Most strains were sensitive to carbendazim,vinclozolin and dimethachlon with the frequency of resistant strains of 5%,2% and 3%, respectively. All B. cinerea strains investigated were susceptible to fenhexamid with the EC50 values ranging from 0.004 μg/mL to 0.2μg/mL.The sensitivity to fenhexamid was proved from the results of analyzing the genotype at the Bc-hch locus. Partial amino acid sequences of β-tubulin and histidine kinase gene showed that point mutation was found to be associated with resistance to these fungicides. The fungicide resistance in populations of B.cinerea in Hubei Province was reported firstly.

关 键 词：灰葡萄孢(Botrytis cinerea) 多菌灵 农利灵 菌核净 环酰菌胺 抗药性 湖北省 

分 类 号：S481.4[农业科学—农药学]