两株野鸭源新城疫病毒分离株F基因和HN基因的分子特性分析  

Molecular Characterization of F Gene and HN Gene of Two Newcastle Disease Virus Isolates from Wild Ducks

作　　者：徐钰[1] 解林红[1] 耿海东[1] 高晓龙[2] 王海军[3] 范胜涛[3] 李元果[4] 王铁成[4] 吴长江[1] 张晓田[1] 孙贺廷[1] 初冬[1] 高玉伟[4] 

机构地区：[1]国家林业局野生动物疫源疫病监测总站,辽宁沈阳110034 [2]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001 [3]吉林农业大学动物科学技术学院,吉林长春130118 [4]国家林业局长春野生动物疫病研究中心,吉林长春130122

出　　处：《动物医学进展》2014年第8期65-69,共5页Progress In Veterinary Medicine

基　　金：国家科技支撑计划项目(2013BAD12B04)

摘　　要：为了分析从湖南长沙分离到的野鸭源新城疫病毒（NDV）融合蛋白基因（F基因）和血凝素-神经氨酸酶基因（HN基因）分子特性,采用RT-PCR方法对分离自野鸭粪便的2株NDV的F基因和HN基因进行了扩增和遗传进化分析。F基因序列分析表明,编码区核苷酸为1 662bp,编码553个氨基酸,F基因碱性裂解位点序列为^112E-R-Q-E-R-L^117,符合NDV弱毒特征,与ClassⅠ类NDV代表毒株核苷酸同源性在92.3%-93.8%之间。HN基因序列分析表明,编码区核苷酸为1 851bp,编码616个氨基酸,与ClassⅠ类NDV代表毒株核苷酸同源性在91.6%-93.9%之间。根据F基因和HN基因遗传进化分析表明,此次分离到的2株NDV同属于ClassⅠ类Genotype 3型。In order to analyze the molecular characteristics of F and HN genes of Newcastle disease virus（NDV）,isolated from wild ducks in Changsha,Hunan province.The F gene and HN gene of two NDV,isolated from feces of wild ducks,were amplified by RT-PCR and genetically analyzed.The nucleotide length of F gene coding region is 1 662 bp,coding 553 amino acids.The motif of cleavage site of the fusion protein was ^112E-R-Q-E-R-L^117,which keeps consistent with the characteristcs of the reference Class I avirulent strains.Homology analysis of F gene revealed that sequence similarities with Class Ⅰ reference strains varied from 92.3%-93.8%.The HN gene contains a 1 851 bp open reading frame,coding 616 amino acids.Homology analysis of HN gene showed 91.6%-93.9%of identities between the two isolates and ClassⅠ reference strains.Phylogenetic analysis of the F gene and HN gene sequences revealed that the two isolates NDV are grouped into genotype 3in ClassⅠ.

关 键 词：新城疫病毒 F基因 HN基因 

分 类 号：S852.659.6[农业科学—基础兽医学] Q789[农业科学—兽医学]