玉米o2和o16突变BC_5F_2近等基因系的选育与遗传背景  

Breeding and Genetic Background of BC5F2 Near-isogenic Lines of o2 and o16 Mutants in Maize

作　　者：王伟 杨文鹏[1,2] 王明春 

机构地区：[1]贵州省旱粮研究所,贵州贵阳550006 [2]贵州省农业生物技术重点实验室,贵州贵阳550006

出　　处：《贵州农业科学》2014年第7期9-12,共4页Guizhou Agricultural Sciences

基　　金：国家自然科学基金"玉米奥帕克-16(o16)突变基因的分离克隆与鉴定"(31160307);贵州省科学技术基金"玉米奥帕克-16突变的蛋白质表达分析"[黔科合J字(2010)2082];贵州省农业科学院专项资金项目"玉米奥帕克-16突变的蛋白质表达分析"[黔农科院院专项(2009)032]

摘　　要：为了探明玉米高赖氨酸奥帕克-2（opaque-2，o2）和奥帕克-16（opaque-16，o16）突变的分子遗传差异，以太系19/o2为o2突变基因供体亲本，QCL3021和 QCL3024为o16突变基因的供体亲本，普通系QCL2179和 QCL2180为受体轮回亲本，构建o2和o16近等基因系（NIL），经过多代回交和自交，并利用SSR分子标记技术。选育近等基因系，并对其进行遗传背景分析。结果表明：多代回交和自交后选育出BC5 F2世代的o2和o16近等基因系（NIL）25份；从40个SSR标记中，筛选出25个在亲本间具有多态性的SSR标记；25个系基于分子标记的遗传背景恢复率为90％～100％，仅有7个系遗传背景恢复率高于理论值（98．4％），表明仍需继续进行回交选择。In order to conduct molecular genetic research on maize high lysine mutants of opaque-2 （o2 ）and opaque-1 6 （o16 ）.The high lysine genes o2 and o16 were backcrossed into normal lines QCL2 1 7 9 and QCL2180 using the maize high lysine inbreds Taixi19 （o2）,and QCL3021（o16）and QCL3024（o16）as donors and normal maize inbred lines QCL2179 and QCL2180 as receptors.Twenty five BC5F2 near-isogenic lines （NILs）of o2 and o16 were bred through multiple backcross and self-cross.Twenty five polymorphic SSR markers between parents among 40 SSR markers were selected to detect genetic background of the NILs.Results：The genetic background recovery rate of 25 NILs was from 90% to 100% based on molecular markers.However,only 7 NILs had higher rate than theoretical value （98.4%）, suggesting that the NILs still need continuous backcross selection.

关 键 词：玉米 OPAQUE-2 opaque-16 近等基因系 育种 遗传背景 

分 类 号：S513[农业科学—作物学]