18个贵州红托竹荪种质资源的遗传多样性  

Genetic Diversity of 18 Dictyophora rubrovolvata Germplasm Resources from Guizhou

作　　者：卢颖颖[1,2] 桂阳[1,2] 龚光禄[1,2] 魏善元[1,2] 朱国胜[1,2] 

机构地区：[1]贵州省农作物品种资源研究所,贵州贵阳550006 [2]贵州省农业生物技术重点实验室,贵州贵阳550006

出　　处：《贵州农业科学》2014年第7期17-20,23,共5页Guizhou Agricultural Sciences

基　　金：贵州省科学技术基金项目"红托竹荪资源遗传多样性研究"[黔科合J字(2011)2169号];贵阳市科学技术计划项目"红托竹荪代料发酵生态循环栽培技术集成与示范"[(2010)筑科农合同字第1-农-17];贵州省科技计划项目"织金竹荪以草代木栽培优良菌株筛选及应用示范"[黔科合NY字(2011)3032]

摘　　要：为红托竹荪优良菌种的选育和开发利用提供参考，利用ITS和ISSR分子标记对18个贵州红托竹荪种质的遗传多样性进行分析。结果表明：1）18个红托竹荪菌株的ITS序列长度为611～621 bp，GC碱基的含量为53．9％～54．68％，相似性为99．9％～100％。2）在遗传相似度为0．77时，将18个红托竹荪菌株分为2个类群。第Ⅰ类群包含13个菌株，分别为3个栽培菌株（ZS017，ZS036，ZS038）和10个野生菌株（YZS010-1，YZS010-2，YZS012，YZS018，YZS033，YZS035，YZS039，YZS041，YZS044，YZS046）；第Ⅱ类群包含5个野生菌株（YZS040，YZS043，YZS045，YZS047，YZS052）。结论，贵州18个红托竹荪种质的亲缘关系较近，没有明显的地域分布特点，其遗传背景差异较小。To provide a reference for breeding and utilizing D.rubrovolvata,the authors analyzed the genetic diversity of D.rubrovolvata strains isolated from Guizhou by ITS and ISSR.Results：1 ）ITS sequence length of 18 D.rubrovolvata strains was 611～621bp,GC base content was 53.9%～53.9% and the similarity was 99.9%～100%.2）The clustering analysis of ISSR showed that these strains could be divided into two groups at a similarity level of 0.77.GroupⅠcontained 13 strains,of which there were three cultivated strains（ZS017,ZS036,ZS038）and 10 wild strains（YZS010-1,YZS010-2,YZS012, YZS018,YZS033,YZS035,YZS039,YZS041,YZS044,YZS046）.GroupⅡ contained five wild strains （YZS040,YZS043,YZS045,YZS047,YZS052 ）.In conclusion,the genetic relationship of these 18 strains was close,and there was not obvious regional distribution characteristics.The genetic background was of little difference.

关 键 词：红托竹荪 内转录间隔区 ITS和ISSR分子标记 遗传多样性 

分 类 号：S646.8[农业科学—蔬菜学]