抗多种病原微生物菌株的分离与鉴定  

Isolation and Identification of Bacterial Strains Resistant to Multiple Pathogenic Microorganisms

作　　者：邵美云[1] 李园园[1] 岳昌武[1] 王苗[1] 

机构地区：[1]遵义医学院医学与生物学研究中心贵州省特色微生物资源及药物开发重点实验室,贵州遵义563003

出　　处：《贵州农业科学》2014年第7期88-92,共5页Guizhou Agricultural Sciences

基　　金：国家自然科学基金"链霉菌卤化产物合成隐形基因簇的克隆与激活"(31160004);贵州省教育厅特色重点实验室建设项目"微生物资源及药物开发"[黔教合KY字(2012)011];贵州省科技学技术基金项目"链霉菌卤化天然产物合成相关基因的克隆与鉴定"[黔科合J字(2010)2156];"链霉菌自诱导肽编码基因簇克隆及功能基因表达"[黔科合J字(2012)2348];"贵州省特殊生境放线菌抗肿瘤活性物质的研究"[黔科合SY字(2013)3013]

摘　　要：为畜牧养殖业新型抗生素药物的研究和开发利用提供优良菌株，采用分离纯化、形态学、分子生物学和超高压液相-紫外二极管阵列-高分辨率质谱联用（UPLC-DAD-HRMS）方法对贵州赤水丹霞地貌区300份土样中的菌株进行分离鉴定。结果表明：1）在分离纯化获得的31株菌株中，有21株菌株对耐万古霉素金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、枯草芽胞杆菌和藤黄微球菌表现出不同的抗菌活性。其中，有8株菌株表现出抗2种测试菌生物活性，1株菌株（S．sp．CSDX435）表现出抗3种测试菌生物活性，1株菌株（S．sp．CSDX416）表现出抗4种测试菌生物活性。2）有20株分离菌株与Stenotrophomonas sp ． A18嗜麦芽窄食单胞菌的16S rRNA同源性达99％，为嗜麦芽窄食单胞菌（Stenotrophononas maltophilia）；11株菌株与S．sp．A18的16S rRNA同源性达78％～97％。其中，S．sp．CSDX58菌株与S．sp．A18的16S rRNA同源性最低，为78％，可能为嗜麦芽窄食单胞菌的新类群。3）从S．sp．CSDX252菌株的发酵代谢产物中发现了可能为聚醚类的新化合物 B，分子式为 C47 H78 O14，且产量较高。因此，在分离得到的31株菌株中，有21株菌株对1种或多种病原微生物表现出不同的抗菌活性，可作为畜禽新型抗生素菌株进行深入研究和开发利用。In order to provide excellent fine strains for research,exploitation and utilization of new antibiotics in livestock breeding industry,bacterial strains in 300 soil samples from Danxia landform area in Guizhou Chishui were isolated and identified by UPLC-DAD-HRMS method.Results：1）31 strains were isolated including 2 1 strains showing various antimicrobial activity to vancomycin-resistant Staphylococcus aureus（VRSA）,methicillin-resistant Staphylococcus aureus （MRSA）,Bacillus subtillis and Micrococcus luteus.Eight strains were resistant to two test bacteria,one resistant to three test bacteria and one resistant to four test bacteria.2）20 isolated strains had 99% 16S rRNA homology with Stenotrophomonas sp.A18,which were identified as Stenotrophononas maltophilia;11 strains had 78%～97% 16S rRNA homology with S.sp.A18.S.sp.CSDX58 had the lowest homology with S.sp.A18,merely 78%,which might be a new population of Stenotrophomonas sp.A new compound B which might be of polyether type was found in the metabolite of S.sp.CSDX252.The molecular formula was C47H78O14,and the yield was high.Consequently,2 1 strains in the 31 isolated strains could be treated as novel antibiotic agents and made further study,development and utilization on them.

关 键 词：病原微生物 分离 鉴定 16SrRNA 抗菌活性 

分 类 号：Q939.11[生物学—微生物学]