水产养殖用地衣芽孢杆菌的分离鉴定与其扩增培养基筛选  

Isolation and Identification of Bacillus licheniformis and Optimization of the Culture Media

作　　者：周冬仁[1] 罗毅志[1] 施伟达[1] 叶雪平[1] 沈振伟[1] 汤美锋 

机构地区：[1]浙江省淡水水产研究所,浙江湖州313001

出　　处：《贵州农业科学》2014年第7期98-100,共3页Guizhou Agricultural Sciences

基　　金：湖州市科技攻关计划项目"中华鳖养殖废水无害化处理技术研究"(2013C102070);浙江省科技厅院所专项"淡水养殖池塘水质调控技术应用与示范"(2013F50014)

摘　　要：为分离筛选适合水产养殖用的地衣芽孢杆菌,并探明不同培养基对其扩增效果,采用微生物学与分子生物学相结合的方法对养殖池塘淤泥中的细菌进行研究。结果表明:分离得到2株抑菌作用最强的菌株DSY002-2011和DSY003-2011,其中,DSY002-2011菌株为地衣芽孢杆菌。由豆粕与红糖、玉米粉、大米粉和麸皮等分别构成的4种生产型培养基均能将DSY002-2011菌株的细菌数扩增至200亿个以上,其中,以红糖20g/L+豆粕80g/L的组合最优,其细菌扩增培养浓度可达39×109 cfu/mL。The combined methods of microbiology and molecular biology were employed to isolate and screen the suitable B.licheniformis for aquaculture and explore the amplification effects of different culture media.Results：Two strains DSY002-2011 and DSY003-2011 with the strongest bacteriostatic action were isolated,in which DSY002-2011 was B.licheniformis,the bacterial count could be up to more than 20 billion in four production media composed of bean pulp,brown sugar,corn flour,rice meal and bran.The combination of browning sugar 20 g/L and bean pulp 80 g/L was of the best effects,the multiplication culture concentration of bacteria was up to 3939×10^9 cfu/mL.

关 键 词：地衣芽孢杆菌 分离鉴定 培养基 细菌扩增 水产养殖 

分 类 号：S154.3[农业科学—土壤学]