黄芪中免疫活性成分的不同提取方式比较  

Comparison of Different Extraction Methods for Immunologically Active Componentsin Astragali Radix

作　　者：陈燕瑞 曾以旺 王少平[1] 代龙[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2014年第16期42-44,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2013ZX09103002-021)

摘　　要：目的:优选黄芪中免疫活性成分的提取工艺。方法:以黄芪甲苷和黄芪总多糖提取量、干浸膏得率为综合评价指标,通过单因素试验考察水提法、1%NaOH温浸法、1%CaO煎煮法对黄芪中免疫活性成分提取效率的影响。采用HPLC测定黄芪甲苷含量,流动相乙腈-水(30∶70),空气泵压力0.4 MPa,ELSD漂移管温度105℃,载气流速2.7 L·min-1;利用UV测定黄芪总多糖含量,检测波长489 nm。结果:3种提取方式的黄芪甲苷提取量分别为0.826,1.016,0.842 mg·g-1,黄芪总多糖提取量分别为14.49,5.82,17.04 mg·g-1,干浸膏得率无显著性差异。结论:采用水提法能达到较好的提取效果,同时生产成本较低,适用于黄芪中免疫活性成分的工业大生产。Objective：To optimize extraction process of immunologically active componentsinAstragali Radix.Method：Taking extracting amounts of astragaloside and total polysaccharides from Astragali Radix,dry extract yieldas comprehensive evaluation index,methods of 1％ NaOH soaking,1％ CaO boiling and water extraction were investigated by single factor test.HPLC was adopted to determine the content of astragaloside with mobile phase of acetonitrile-water （30 ∶ 70）,air pump pressure of 0.4 MPa,ELSD drift tube temperature at 105 ℃ and carrier gas flow rate of 2.7 L ·min-1 ; the content of total polysaccharides from Astragali Radix was determined by UV,detection wavelength was set at 489 nm.Result：By extracting of these three kinds of methods,extracting amounts ofastragaloside were 0.826,1.016,0.842 mg · g-1,extracting amounts oftotal polysaccharides were 14.49,5.82,17.04 mg· g-1,respectively; but dry extract yield had no significant difference.Conclusion：Water extraction process could achieve better extraction effect by comparing with the other two methods,itcould reduce production costs for industrial mass production of immunologically active componeotsinAstragali Radix.

关 键 词：黄芪 黄芪甲苷 总多糖 提取方法 干浸膏得率 

分 类 号：R283.6[医药卫生—中药学]