蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的测定  

Content Determination of Chlorovaltrate and Valjatrate B in Total Iridoids of Valerianae Jatamansi Rhizoma et Radix

作　　者：许科科 李少华[1] 左长英[1] 陈朝勇[1] 张瑞桐[1] 兰明[1] 林玉[1] 张天娥[2] 闫智勇[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]成都中医药大学基础医学院,成都611137

出　　处：《中国实验方剂学杂志》2014年第16期64-66,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-370);教育部中央高校基本科研业务费专项资金(WJTU09CX063)

摘　　要：目的：运用高效液相色谱法测定蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的含量.方法：采用高效液相色谱法,waters C18色谱柱（4.6 mm×250 mm,5μm）;chlorovaltrate和蜘蛛香素B的流动相分别为乙腈-水（60∶40）,（50：50）;检测波长分别为254,203 nm;流速均为1.0 mL·min-1;柱温均为30℃.结果：蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B进样量分别在15.5 ～155,20 ～200 pg与各自峰面积积分值呈良好线性关系（r分别为0.998 6,0.998 7）;测得chlorovaltrate和蜘蛛香素B含量分别为8.91,7.00 mg·g-1.结论：确定了蜘蛛香总环烯醚萜中chlorovaltrate和蜘蛛香素B的含量,为蜘蛛香总环烯醚萜的质量控制提供了一定依据.Objective：The purpose of this study was to determine the content of chlorovaltrate and valjatrate B in total Iridoids of Valerianae Jatamansi Rhizoma et Radix.Method：HPLC method was adopted.The chromatographic column was waters C1s column （4.6 mm ×250 mm,5 μm） ; the mobile phase for chlorovaltrate and valjatrate B was acetonitrile water （60∶40）,（50∶ 40）; the detection wavelength for chlorovaltrate and valjatrate B were 254,203 nm; the flow rate was 1.0 mL ·min-1 ; the column temperature was 30 ℃.Result：The fine linear correlation displayed when the injection volumn were 15.5-155 pg （r =0.998 6）,20-200 pg （r =0.998 7） for chlorovaltrate and valjatrate B in total iridoids of Valerianae Jatamansi Rhizoma et Radix,respectively.The contents of chlorovaltrate and valjatrate B were 8.91 mg ·g-1 and 7.00 mg ·g-1,respectively.Conclusion：The contents of chlorovaltrate and valjatrate B in total Iridoid of Valerianae Jatamansi Rhizoma et Radix were determined to provide evidence for quality control of total iridoids of Valerianae Jatamansi Rhizoma et Radix.

关 键 词：蜘蛛香总环烯醚萜 chlorovaltrate 蜘蛛香素B 

分 类 号：R284.1[医药卫生—中药学]