HPLC测定新疆紫草中紫草素的含量及提取方法的优化  

Ultrasonic-assisted Extraction and Content Determination of Shikonin from Arnebiae Radix by HPLC

作　　者：崔晓秋[1] 刘云刚 

机构地区：[1]济宁医学院药学院,山东日照276826 [2]岚山生化有限公司,山东日照276800

出　　处：《中国实验方剂学杂志》2014年第16期94-97,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：济宁医学院青年基金项目(JYQ2011KM027)

摘　　要：目的：采用超声方法提取新疆紫草中紫草素,并以左旋紫草素为指标,建立HPLC测定紫草素的含量.方法：利用超声波协同甲醇提取40 min;色谱条件为Shim-pack CLC-ODS-18色谱柱（4.6 mm ×250 mm,10 μm）,检测波长516 nm,流动相甲醇-水（85∶15）,流速1.0 mL·min-1,柱温室温.结果：左旋紫草素进样量在0.127～1.143 μg具有良好的线性关系,回归方程为y=338 763X-198 347（r =0.999 8）,平均回收率99.3％,RSD 1.18％,左旋紫草素超声法提取率是回流提取法的1.29倍.结论：经方法学验证,该实验所采用的实验方法重复性好、稳定、准确、可靠,可用于中药紫草药材中紫草素的含量测定.Objective：To develop a method for ultrasonic-assisted extraction and high performance liquid chromatography （HPLC） determination of shikonin in the roots of Arnebiae Radix.Method：Shikonin was extracted by ultrasonic-assisted-methyl alcohol method.The HPLC determination condition consisted of Shim-pack CLC-ODS-18 （4.6 mm × 250 mm,10 μm） and shikonin was detected at 516 nm wavelength by using methyl alcohol-water （85∶15） as the mobile phase,column temperature at room temperature and flow rate 1.0 mL · min-1.Result：Shikonin had a good linearity relation in the range 0.127-1.143 μg （r =0.999 8）,regressive equation were Y=338 763X-198 347.The average recovery was 99.3％,RSD 1.18％ （n =6）.In contrast with refluxing process,the extraction efficiency of ultrasonic extraction was 1.29 times.Conclusion：The methods of ultrasonic extraction and HPLC determination were proved to be accurate,reliable and repeatable.It can be used to determine natural shikonin content of Arnebiae Radix easily.

关 键 词：左旋紫草素 高效液相色谱 超声波辅助提取 紫草 

分 类 号：R284.1[医药卫生—中药学]