丹栀逍遥散6种有效成分的HPLC测定  

Simultaneous Determination of Six Constituents in Danzhi Xiaoyaosan by HPLC

作　　者：杨永红 

机构地区：[1]贵州省骨科医院药剂科,贵阳550007

出　　处：《中国实验方剂学杂志》2014年第16期97-99,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立丹栀逍遥散制剂中有效成分栀子苷、中京尼平苷、芍药内酯苷、甘草甜素、芍药苷以及牡丹酚6种成分的测定分析方法。方法:采用HPLC,Cosmosil 5C18色谱柱,(4.6 mm×250 mm,5μm),流动相乙腈-0.5%磷酸水(15∶85)梯度洗脱,流速1.0 mL·min-1,波长230 nm。结果:根据上述方法可在90 min内成功地分离丹栀逍遥散中栀子苷、京尼平苷、芍药内酯苷、甘草甜素、芍药苷以及牡丹酚6个指标成分,在丹栀逍遥散中各成分质量分数为栀子苷1.203 mg·g-1,京尼平苷1.960 mg·g-1,芍药内酯苷6.720 mg·g-1,甘草甜素0.157 mg·g-1,芍药苷0.012 mg·g-1,牡丹酚0.127 mg·g-1。结论:该方法可用于丹栀逍遥散中栀子苷、京尼平苷、芍药内酯苷、甘草甜素、芍药苷以及牡丹酚的测定,操作简单、结果准确、回收率高、重复性好,可用于丹栀逍遥散及其相关制剂的质量控制。Objective：To establish a method for simultaneous determination of gardenin,geniposide,albiflorin,paeoniflorin,liquiritigenin,paeonol in Danzhi Xiaoyaosan by HPLC.Method：The Cosmosil 5 C18 column （4.6 mm × 250 mm,5 μm） was used with a mobile phase of acetonitrile （A）-0.5 ％ phosphoric acid solution （B） gradient elution,the flow rate was 1 mL ·min-1,and the detection wave-lengths were set at 230 nm.Result：The linear ranges were 0.009 46-0.946 mg ·L-1 for gardenin,0.009 58-0.958 mg ·L-1 for geniposide,0.008 68-0.868 mg · L-1 for albiflorin,0.004 88-0.488 mg · L-1 for paeoniflorin,25.0-500 mg · L-1 for liquiritigenin,25.0-500 mg · L-1 for paeonol respectively.The average recoveries of three constituents were 96.1％,104.3％,100.5％,94.7％,97.4％,95.4％ with the RSD of 1.32％,2.55％,0.67％,1.29％,1.36％,2.38％.The contents of six compounds,gardenin,geniposide,albiflorin,paeoniflorin,liquiritigenin,paeonol in Danzhi Xiaoyaosan were 1.203,0.960,0.720,0.157,0.104,0.127 mg · g-1.Conclusion：Determination of Danzhi Xiaoyaosan could effectively control the product quality of Danzhi Xiaoyaosan.

关 键 词：丹栀逍遥散 栀子苷 京尼平苷 芍药内酯苷 甘草甜素 芍药苷 牡丹酚 

分 类 号：R284.1[医药卫生—中药学]