莱菔子提取液中萝卜苷的大鼠肠吸收特性考察  

Investigation of Intestinal Absorption Characteristics in Rats of Glucoraphenin in Raphani Semen Extract

作　　者：朱立俏[1] 盛华刚[1] 周洪雷[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2014年第16期124-126,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省自然科学基金项目(ZR2010HM017);山东省高等学校科技计划项目(J105LL05);山东中医药大学校级课题(ZYDXY1332)

摘　　要：目的:考察莱菔子水提液中萝卜苷在大鼠小肠的吸收特性。方法:采用大鼠在体单向肠灌流法考察不同药物质量浓度(0.02,0.05,0.1 g·mL-1)对萝卜苷肠吸收特性的影响,计算吸收速率常数(Ka)和表观吸收系数(Papp)。采用HPLC测定萝卜苷含量,流动相0.1%磷酸溶液-乙腈(87∶13),检测波长225 nm。结果:萝卜苷在pH 5.12的K-R试液中稳定性最好。不同质量浓度的莱菔子水提液中萝卜苷在大鼠小肠的Ka分别为(7.750±1.744)×10-3,(7.204±1.045)×10-3,(7.148±0.837)×10-3min-1,Papp分别为(1.519±0.507)×10-3,(1.504±0.617)×10-3,(1.592±0.345)×10-3cm·min-1,均无显著性变化。结论:萝卜苷为易吸收成分,在大鼠小肠中的吸收机制为被动扩散。Objective：To investigate small intestine absorption characteristics in rats of glucoraphenin in Raphani Semen extract.Method：Taking in situ rat single-pass intestinal perfusion model,effects of different drug mass concentrations （0.02,0.05,0.1 g ·mL-1） on intestinal absorption characteristics of glucoraphenin was investigated,and calculated their absorption rate constant （Ka） and apparent absorption coefficient （Papp）.HPLC was employed to determine the content of glucoraphenin with mobile phase of 0.1％ phosphoric acid solutionacetonitrile （87∶ 13） and detectopm wavelength at 225 nm.Result：Glucoraphenin had the best stability in K-R test solution with pH of 5.12.In Raphani Semen extract with different concentrations,Ka of glucoraphenin in small intestine of rats were （7.750±1.744） ×10-3 （7.204 ±1.045） ×10-3 （7.148 ±0.837） ×10-3 min-1 Papp were （1.519 ±0.507） ×10-3,（1.504 ±0.617） ×10-3,（1.592 ±0.345） ×10-3 cm.min-1,these data had no significant changes.Conclusion：Glucoraphenin was easy-to-absorped component,its absorption mechanism in small intestine of rats was passive diffusion.

关 键 词：莱菔子 萝卜苷 在体单向肠灌流模型 肠吸收特性 硫苷类成分 

分 类 号：R283.1[医药卫生—中药学] R283.6[医药卫生—中医学]