川芎嗪微乳凝胶预防大鼠实验性腹腔粘连抗炎及抗氧化作用  

Anti-inflamamatory and Anti-peroxidation Action of Ligustrazine Microemulsion Gel in Ankylenteron Rats

作　　者：张慧[1] 宋宇[1] 张婷[2] 曾莉[3] 

机构地区：[1]新乡医学院药学院药理学教研室,河南新乡453000 [2]新乡医学院三全学院,河南新乡453003 [3]南京中医药大学,南京210023

出　　处：《中国实验方剂学杂志》2014年第16期159-164,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省2012年度普通高校研究生科研创新计划项(CXZZ12_0620);南京中医药大学基础研究与重点培育项目(09XPY05)

摘　　要：目的:观察川芎嗪微乳凝胶对肠粘连的防治效果,通过检测大鼠腹腔液细胞因子及粘连组织抗氧化酶进一步探讨其对肠粘连防治作用的机理。方法:取SD大鼠70只,随机分为7组:正常组、模型组、川芎嗪对照组、空白微乳凝胶组,川芎嗪微乳凝胶高、中、低剂量组,每组10只。除正常对照组外,其余各组大鼠均制备肠粘连模型。正常对照组和模型组ip生理盐水,川芎嗪对照组ip川芎嗪注射液(15 mg·kg-1·d-1),连续给药10 d。川芎嗪微乳凝胶低、中、高剂量组(7.5,15,30 mg·kg-1)在造模后,腹腔内以注射器均匀涂抹于创面,并按10 d量放大,一次性给药。各组大鼠于术后第11天处死,ELISA法测定腹腔液白介素-6(IL-6)、白介素-18(IL-18)和肿瘤坏死因子α(TNF-α)含量,并取粘连组织检测超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)、还原型谷胱甘肽(GSH)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活力及丙二醛(MDA)、一氧化氮(NO)含量,同时记录大鼠肠粘连级别。取粘连肠组织观察病理变化。结果:与正常组相比,模型组腹腔液IL-6,IL-18和TNF-α含量增高(P<0.01),粘连组织中MDA,NO,NOS活性增强(P<0.01),CAT,GPx,GSH,SOD的活性下降(P<0.01)。与模型组相比,川芎嗪微乳凝胶低、中、高剂量组的肠粘连程度明显减轻(P<0.05,P<0.01),病理显示肠粘连逐渐松解,腹腔液IL-6,IL-18和TNF-α含量明显降低(P<0.05,P<0.01),粘连组织中MDA,NO含量及NOS活性明显下降(P<0.05,P<0.01),CAT,GPx,GSH,SOD的活性增强(P<0.05,P<0.01)。结论:川芎嗪微乳凝胶可明显减轻肠粘连的程度,对肠粘连具有防治作用。其作用可能是通过下调IL-18的表达,进而使相关基因的表达下调,导致下游炎性因子TNF-α,IL-6等表达减少,同时与抗肠组织过氧化损伤有关。Objective：To investigate the effect of ligustrazine microemulsion gel on the expression of ctokine in peritoneal fluid and antioxidase in the ahension tissue in rats with peritoneal adhesion.Method：Seventy SD rats were randomly divided into 7 groups：normal control group,model group,blank microemulsion gel groups （5 mL· kg-1）,ligustrazine group （15 mg ·kg-1） and low-,medium-and high-dose ligustrazine microemulsion gel groups （in the dose of 7.5,15,30 mg · kg-1 respectively）.Except the normal control group,the rats in other groups were induced ankylenteron.Intraperitoneal injection of saline was administered to the rats in the normal control group and the model group,and ligustrazine groups were given ligustrazine injection to abdominal cavity.The treatment lasted 10 days.In the low-,medium-and high-dose ligustrazine microemulsion gel groups,the microemulsion gel were scribbled evenly on the wound by syringe in the abdominal cavity after modeling.On the 11 th day after surgery,the peritoneal fluid and the adhesion tissue of each group were collected.The levels of interleukin-18 （IL-18）,interleukin-6 （IL-6） and tumor necrosis factor-a （TNF-a） in peritoneal fluid were determined by enzyme linked immunosorbent assay and the contents of superoxide dismutase （SOD）,nitric oxide synthase （NOS）,glutathione （GSH）,Malondialdehyde （MDA）,nitric oxide （NO）,catalase （CAT）,glutathione peroxidase （GPx） in the adhesion tissue were measured by kits.Grades of intestinal adhesion were ranked by macroscopic observation.The intestine tissue were adopted to observe the change of pathomorphology.Result：Comared with the contol group,ligustrazine microemulsion gel could significantly relieve the experimental intestinal adhesion and decreased the levels of IL-18,IL-6 and TNF-α in peritoneal fluid obviously （P ＜ 0.05,P ＜ 0.01）.The activity of MDA,NO,NOS in the adhesion tissue were reduced significantly （P ＜0.05,P ＜0.01）,but the activity of CAT,GPx,GSH,SOD were inc

关 键 词：川芎嗪 微乳凝胶 肠粘连 白介素-6 白介素-18 肿瘤坏死因子α 氧化损伤 

分 类 号：R285.5[医药卫生—中药学]