MicroRNA-150通过降低CD122的表达调控NK及NKT细胞的分化  被引量:1

MicroRNA-150 regulates differentiation of NK and NKT cells through decre-asing CD122 expression

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作  者:张爱红[1] 郑全辉[2] 郑建兴[1] 李娟[2] 侯志宏[2] 刘亚楠[2] 张庆波[2] 

机构地区:[1]唐山市工人医院,唐山063000 [2]河北联合大学,唐山063000

出  处:《中国免疫学杂志》2014年第8期1009-1014,共6页Chinese Journal of Immunology

基  金:国家自然科学基金面上项目(No.81373111);河北省自然科学基金面上项目(No.H2013209019);唐山市科技局指令性项目(No.13130290z)资助

摘  要:目的:探讨microRNA-150缺失对调节性T细胞(Treg)、γδT细胞、NK及NKT细胞产生、发育和自稳的影响。方法:采用microRNA-150基因敲除小鼠;Real-time PCR检测microRNA-150的表达;流式细胞术检测小鼠胸腺和脾脏Treg、γδT细胞、NK及NKT细胞数量变化;采用Annexin V染色法检测细胞凋亡;5-溴脱氧尿嘧啶核苷(Brdu)掺入法检测细胞增殖。结果:microRNA-150缺失对小鼠Treg和γδT细胞的产生和发育不发挥明显作用,但导致NK和胸腺NKT细胞数量显著降低,而且,microRNA-150缺失导致小鼠NK及NKT细胞中CD122的表达显著降低,细胞发育受阻,凋亡增加,同时NK细胞的增殖能力也显著降低。结论:microRNA-150调控小鼠NK和NKT细胞的发育和自稳,CD122可能在以上调控过程中发挥重要作用。To explore the effects of microRNA-150 deletion on the development and homeostasis of regulatory T cells (Treg),γδT cells,NK and NKT cells.Methods:microRNA-150 knockout mice were used and microRNA-150 expression was detected by Real-time PCR.The numbers of Treg ,γδT,NK and NKT cells in the thymus and spleen of normal control and microRNA-150 knockout mice were detected by Flow cytometry.Cell apoptosis was detected by Annexin V staining , and cell proliferation was detected by 5-Bromo-2-deoxyUridine ( Brdu ) incorporation.Results: microRNA-150 deletion did not affect the development and homeostasis of regulatory T cells (Treg) andγδT cells.However,microRNA-150 deletion resulted in a significant reduction of the NK and thymic NKT cell number.In addition, microRNA-150 deleted NK and NKT cells showed an arrested developmental and maturational phenotype with a reduced expression of NK 1.1 and CD122.Moreover , cell apoptosis was significantly increased in microRNA-150 deleted NK and thymic NKT cells ,while a lower cell proliferation rate was shown in the microRNA-150 deleted NK but not NKT cells.Conclusion: CD122 may play an important role in the development and homeostasis of mouse NK and NKT cells regulated by microRNA-150.

关 键 词:microRNA-150 CD122 NK NKT细胞 

分 类 号:R310.34[医药卫生—基础医学]

 

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