子宫内膜异位症不孕与CD4^+CD25^+FoxP3调节性T淋巴细胞的相关性研究  

Relationship between CD4^+CD25^+FoxP3 regulatory T cells and endometriosis associated infertility

作　　者：梁艳[1] 陈淑芳[1] 张健[1] 

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院,上海200010

出　　处：《上海交通大学学报（医学版）》2014年第7期997-1000,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市卫生局青年科学研究项目(2010Y1)~~

摘　　要：目的探讨子宫内膜异位症(EM)不孕与围种植期外周血及在位内膜CD4+CD25+FoxP3调节性T淋巴细胞(Tregs)变化的相关性。方法采用流式细胞术检测27例不同期别EM不孕患者外周血单个核细胞CD4+CD25+FoxP3 Tregs数量及其占CD4+T淋巴细胞的百分比,RT-PCR检测在位内膜CD4+CD25+FoxP3mRNA表达水平。以已生育非EM女性作为对照组(n=20)。结果与对照组比较,轻度EM不孕组外周血单个核细胞中CD4+CD25+FoxP3 Tregs数量显著增多,其占CD4+T淋巴细胞百分比增高,差异均有统计学意义(P<0.05);重度EM不孕组CD4+CD25+FoxP3 Tregs数量及其占CD4+T淋巴细胞百分比均显著高于轻度EM不孕组,差异均有统计学意义(P<0.05)。在位内膜FoxP3 mRNA相对表达量检测结果显示,轻度EM不孕组显著高于对照组(P=0.000 1),重度EM组显著高于轻度EM组和对照组(P<0.000 1)。结论 EM不孕围种植期CD4+CD25+FoxP3 Tregs与内膜容受性异常导致不孕的机制无直接关系;CD4+CD25+FoxP3Tregs可能与EM的发生和发展有关,可能加重EM所致病变而导致不孕。Objective To explore the relationship between the endometriosis （EM） associated infertility and changes of CD4^＋ CD25^＋ FoxP3 Tregs in the peripheral blood and eutopic endometrium during peri-implantation phase. Methods Twenty-seven women with EM associated infertility of different stages were selected. The CD4^＋ CD25^＋ FoxP3 Tregs counts in peripheral blood mononulear cells （PBMCs） and the ratio of CD4^＋ T ceUs and PBMCs were detected by the flow cytometry. The expression of CD4^＋ CD25^＋ FoxP3 mRNA of eutopic endometrium was detected by the RT-PCR. The controls were twenty procreated women without EM （n =20）. Results Compared to the controls, the CD4 ^＋ CD25^＋ FoxP3 Tregs counts in PBMCs and the ratio of CD4 ^＋ T cells and PBMCs of women with minor EM were significantly higher. The differences were statistically significant （P〈0.05）. The CD4 ^＋ CD25 ^＋ FoxP3 Tregs counts in PBMCs and the ratio of CD4^＋ T cells and PBMCs of women with severe EM were significantly higher than those of women with minor EM. The differences were statistically significant （P〈0.05） . The relative expression of FoxP3 mRNA of eutopic endometrium showed that the expression of women with minor EM was significantly higher than that of controls （P=0. 000 1）, and the expression of women with severe EM was significantly higher than that of controls and women with minor EM （P〈0. 000 1）. Conclusion The CD4^＋ CD25^＋ FoxP3 Tregs in the periimplantation phase are not directly relevant to the pathogenesis of abnormal endometrial receptivity of infertile women with EM. CD4^＋ CD25^＋FoxP3 Tregs may be relevant to the incidence and development of EM and may aggravate lesions caused by EM and lead to the infertility.

关 键 词：子宫内膜异位症 CD4^+CD25^+FoxP3 不孕症 

分 类 号：R711.71[医药卫生—妇产科学]