HPLC法测定人血浆中奥沙普秦含量及其药物动力学  

Determination and pharmacokinetic of oxaprozin in human plasma by HPLC

作　　者：谢伟 关博 

机构地区：[1]西宁市第二人民医院,青海西宁810003 [2]辽宁省金秋医院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第8期649-653,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立HPLC法测定人血浆中奥沙普秦（oxaprozin,Oxa）浓度的方法,并研究其在健康受试者体内的药物动力学。方法采用固相萃取法提取样品,以布洛芬为内标物,采用Diamonsil-C18（150 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-20 mmol·L-1醋酸铵水溶液（pH=3.0±0.2）（体积比为63∶27）,流速为1.0 mL·min-1,柱温为30℃,检测波长为280 nm。结果 Oxa在0.5~150.0 mg·L-1内与峰面积呈良好线性关系（r=0.996 9）,日间和日内精密度RSD均小于6.5%,提取回收率大于68.4%。主要药物动力学参数为：ρmax（84±12）mg·L-1,tmax（3.2±1.4）h,t1/2（49±4）h,AUC0→t（2 383±327）mg·h·L-1,AUC0→∞（2 468±346）mg·h·L-1。结论 HPLC法适用于临床测定奥沙普秦在人体中的浓度。Objective To develop an HPLC method for determination of oxaprozin（Oxa） in the healthy sub-jects plasma, and study its pharmacokinetics after oral administration of oxaprozin dispersible tablets. Meth- ods Solid phase extraction method was used to extract the sample. Separation was achieved on a reversed- phase C18 column with a mobile phase of methanol-20 mmol·L-1 ammonium acetate solution（pH =3.0 ±0. 2 ） （ V： V = 63： 27 ）. The flow rate was 1.0 mL· min - 1 and the column temperature was 30 ℃. Ibuprofen was used as an internal standard. Results The calibration curve of Oxa was linear in the concentration ranges of 0. 5-150. 0 mg.L-~ （ r =0. 996 9）. The RSDs of inter-and intra-day were less than 6.5% and the average recovery of Oxa was more than 68.4%. The main pharmacokinetics parameters of Oxa were as follows ：Pmax （84 ± 12） mg·L-1 , tmax（3.2 ± 1.4） h,t1/2（49 ±4） h,AUC0→t,（2 383 ±327） mg-h.L-1 ,AUC0→∞ （2 468 ± 346 ） mg. h-L-l. Conclusions This method is simple, quick with good reproducibility, and was suitable for pharmacokinetic study of oxaprozin in healthy subjects.

关 键 词：奥沙普秦 健康受试者 药物动力学 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]