凉血解毒汤对寻常型银屑病血热证患者外周血Th17细胞相关细胞因子的影响  

Effects of Liangxue Jiedu decoction on Th17 associated cytokines in peripheral blood mononuclear cells of patients with blood-heat syndrome of psoriasis vulgaris

作　　者：田海刚[1] 胡帅 尤立平[3] 宋佩华[3] 向青[4] 李红艳[4] 徐波[4] 张晓艳[3] 

机构地区：[1]北京中医药大学,北京100029 [2]解放军159医院,河南驻马店463000 [3]中日友好医院皮肤科,北京100029 [4]中日友好医院临床研究所,北京100029

出　　处：《中日友好医院学报》2014年第4期212-215,共4页Journal of China-Japan Friendship Hospital

基　　金：国家自然科学基金面上项目(81171502)

摘　　要：目的:探讨凉血解毒汤治疗寻常型银屑病血热证的作用机制。方法:30例寻常型银屑病血热证患者和18例健康人为受试对象,其中给予10例中重度患者凉血解毒汤治疗6周,所有受试者分别于治疗前(W0)采集外周血,10例患者治疗6周(W6)再次采集外周血,分离所有治疗前后外周血单个核细胞,提取总RNA进行实时定量PCR检测,分析外周血单一核细胞(PBMC)中Thl7细胞相关因子IL-17A、IL-17F、IL-21、IL-22 mRNA的相对表达量。结果:患者组PBMC中Thl7细胞因子IL-17A、IL-17F、IL-21、IL-22 mRNA相对表达量均显著高于对照组(均P<0.05);凉血解毒汤治疗6周后,患者PBMC中Thl7细胞因子IL-17A、IL-17F、IL-21、IL-22mRNA相对表达量较治疗前均显著降低(均P<0.05)。结论:凉血解毒汤可能通过抑制Th17细胞的活化及其效应细胞因子的分泌,下调17A、IL-17F、IL-21、IL-22 mRNA水平,从而有效治疗血热证银屑病。Objective：To investigate the therapeutic mechanism of Liangxue Jiedu Decoction on blood-heat syndrome of psoriasis vulgaris.Methods：Thirty patients with blood-heat syndrome of psoriasis vulgaris and 18 healthy individuals were enrolled,and 10 patients with moderate to severe psoriasis were treated by Liangxue Jiedu Decoction for a eonsective 6 weeks.Peripheral blood of all subjects were collected before treatment （W0）,Peripheral blood of 10 patients who received therapy were collected again after treatment of 6 weeks （W6）.Peripheral blood monocytes of W0 and W6 were isolated and the total RNA was extracted for perform ing Quantitative real-time reverse transcriptase polymerase chain reaction （RT-PCR） assay to analyse expres sion levels of Thl7 cytokines （IL-17A,IL-17F,IL-21,IL-22）in peripheral blood mononuclear ceils （PBMCs）. Results：The mRNA expression levels of these eytokines in PBMC of patient group were significantly increased compared with that of healthy subjects （all P〈0.05）.And the mRNA expression levels of post-treatment were significantly down-regulated compared with that of pre-treatment （allP〈0.05）.Conelusion：The study indicates the therapeutic mechanism of Liangxue Jiedu Decoction on psoriasis of blood-heat syndrome was likely asso ciated with a potential down-regulation of Thl7 cytokines （IL-17A,IL-17F,IL-21,IL-22）mRNA level through inhibiting the activation of Thl7 cells and production of effector cytokines.

关 键 词：寻常型银屑病 血热证 凉血解毒汤 T辅助细胞-17 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]