捻转补泻手法对自发性高血压大鼠左心室组织形态心肌内皮素-1及心肌胶原mRNA表达的影响  

Effects of twirling-rotating reinforcing and reducing technique for left ventricular morphology,concentration of ET-1 and expression of TypeⅠ,Ⅲ collagen mRNA in spontaneous hypertensive rats

作　　者：张英英[1] 刘清国[1] 徐萌[1] 郭妍[1] 刘金艳[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中国针灸》2014年第8期791-797,共7页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:81072861

摘　　要：目的:探讨针刺捻转补法和捻转泻法干预对自发性高血压大鼠(spontaneous hypertensive rats,SHR)心肌损害的效应差异。方法:将60只雄性11周SHR大鼠随机分为4组,即模型对照组(A)、捻转补法组(B)、捻转泻法组(C)、针刺留针组(D),每组15只,并以11周龄雄性Wistar大鼠12只作为空白对照组(E)。A组和E组不针刺,进行与其他组相同的抓捉捆绑刺激;B组针"太冲"穴,施捻转补法1min,留针9min;C组针"太冲"穴,施捻转泻法1min,留针9min;D组针"太冲"穴,不予以手法,留针10min,每天针刺1次,针刺6d休息1d,一共针刺24d。针刺前和针刺开始后每隔6天用无创血压仪测量大鼠收缩压和舒张压;28d后苏木精-伊红(HE)和三色(Masson)染色法观察左心室肥大、增生及心肌纤维化情况;酶联免疫法(Elisa)测定左心室心肌内皮素-1(ET-1)含量;PCR半定量法分析左心室肌Ⅰ、Ⅲ型胶原mRNA表达变化。结果:(1)血压:实验后A、B两组的收缩压和舒张压均有升高(P<0.05),C、D两组收缩压和舒张压均降低(P<0.05),C组的降压效应较D组更明显(P<0.05)。(2)HE和Masson染色显示:除E组外,A、B、C、D组均有心肌肥大和心肌纤维化,C组最轻,其次为D组,A组和B组均比较严重。(3)ET-1浓度:5组间ET-1浓度均有差异(均P<0.05),浓度由高到低依次为:A、B、D、C、E组。(4)Ⅰ型胶原mRNA:C组与D组的Ⅰ型胶原mRNA水平比较差异无统计学意义(P>0.05),C组与A、B组相比,水平较低(均P<0.05),E组的胶原水平最低;Ⅲ型胶原mRNA:A组与B组比较差异无统计学意义(P>0.05),C组与A、B、D组相比,C组的Ⅲ型胶原mRNA水平较低(P<0.05)。结论:捻转泻法可以降低SHR的收缩压和舒张压,能有效抑制ET-1的合成和Ⅰ、Ⅲ型胶原mRNA的表达,对Ⅲ型胶原mRNA抑制作用更明显,与捻转补法存在生物效应差异。Objective To explore the effect differences between twirling-rotating reinforcing and reducing tech- nique of acupuncture on cardiac damage in spontaneous hypertensive rats （SHR）. Methods Sixty male ll-week- old SHR were randomly divided into four groups： a model control group （group A）, a twirling-rotating reinforcing technique group （group B）, a twirling-rotating reducing technique group （group C） and a needle retaining group （group D）, 15 rats in each one. In addition, twelve male ll-week-old Wistar rats were used as a blank control group （group E）. Acupuncture was not used in group A and group E, only with grasp, capture and binding stimulation that was also adapted in the rest groups. Rats in the group B were treated with acupuncture at ＂Taichong＂ （LR 3） by twirling^ro- rating reinforcing technique for 1 rain and then the needles were retained for 9 mira rats in the group C were treated with acupuncture at ＂Taichong＂ （LR 3） by twirling-rotating reducing technique for 1 min and then the needles were retained for 9 mira rats in the group D were treated with acupuncture at ＂Taichong＂ （LR 3） hut without any technique and then needles were retained for 10 min. Before and after acupuncture, blood pressure monitor was used to measure therats＇ systolic pressure and diastolic pressure every 6 days. Twenty-eight days after the treatment, HE and Masson staining were adopted to observe the status of left ventricular hypertrophy and myocardial fibrosis. ELISA method was applied to test the content of endothelin-1 （ET-1）. PCR semiquantitative method was used to analyze Type I and HI collagen mRNA in the left ventricular. Results （1） Blood pressure., after the treatment, the systolic pres- sure and diastolic pressure were both increased in the group A and the group B （P〈0.05） ; while the two pres- sures were both lowered in the group C and the group D （P〈0. 05）, which was more obvious in the group C （P〈0. 05）. （2） Aecording to HE and Masson s

关 键 词：自发性高血压 捻转补法 捻转泻法 心肌损害 内皮素-1(ET-1) I Ⅲ型胶原mRNA 

分 类 号：R246.1[医药卫生—针灸推拿学]