淫羊藿总黄酮对实验性糖尿病大鼠肾脏保护作用  

Protective effect of total flavonoids of epimedium on the kidney in experimental diabetic rats

作　　者：钱虹[1] 杨钧杰[1] 潘定一[1] 唐文涛[1] 许可佳[1] 齐敏友[1] 

机构地区：[1]浙江工业大学药学院,杭州310014

出　　处：《中国应用生理学杂志》2014年第4期314-317,共4页Chinese Journal of Applied Physiology

基　　金：浙江省教育厅重点课题(Z201121669);浙江省新苗人才计划(2013R403050)

摘　　要：目的:研究淫羊藿总黄酮(TFE)对链脲佐菌素(STZ)致糖尿病大鼠肾脏损伤的影响,并初步探讨其可能的作用机制。方法:健康雄性SD大鼠一次性尾静脉注射STZ(40 mg/kg)建立糖尿病模型。动物随机分成3组(n=10):对照组、模型组和TFE组(100 mg/kg,i.g.)。12周后,处死大鼠。测定空腹血糖,肾脏脏器系数,血清尿素氮(BUN)、肌酐(Cr)含量;测定肾组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;Masson染色观察肾组织胶原纤维增生;免疫组化测定转化生长因子β1(TGF-β1)蛋白的表达。结果:与对照组比较,模型组肾脏脏器系数增大、肾功能下降、肾组织抗氧化能力降低;病理学可见肾小球、肾小管间质纤维化;同时TGF-β1蛋白表达水平上调。TFE组明显改善上述指标。结论:TFE对STZ致糖尿病大鼠肾脏损伤有明显的改善作用,其作用机制可能与抗氧化作用和抑制TGF-β1蛋白表达有关。Objective： To investigate the influence of total flavonoids of epimedium（TFE） on the streptozocin（STZ）-induced kidney injury in diabetic rats and discuss the possible mechanism. Methods： Diabetes was produced by a single injection of streptozocin （40 mg/kg, iv） in male SD rats. The rats were randomly divided into three groups（ n = 10） ： control group, model group and TFE group （100 mg/kg, ig）. Animals were sacrificed 12 weeks later. The level of blood glucose, blood urea nitrogen（BUN） and ereatinine（Cr） as well as the renal index were determined. Detect the specific biochemical of renal tissue： supemxide dismutase（SOD）, malondialdehyde （MDA）. Use masson staining to observe the morphology of the renal tissue, lmmunohistochemistry was employed to determine the protein levels of transfonning growth factor-β1 （TGF-β1）. Results： Compared to control group, the enhancement of blood glucose, renal index, BUN and Cr was found in model group, which was significantly attenuated by treatment with TFE. Meanwhile, elevated MDA level in renal tissue as well as decreased SOD activities in renal tissue were significantly remitted by TFE. Furthermore, TFE decreased the expression of TGF-β1. Conclusion： TFE can evidently relieve renal damage in rots with diabetic nephropathy induced by SIZ, which might be related to antioxidation and modulating the expression of TGF-β1 protein.

关 键 词：淫羊藿总黄酮 糖尿病肾病 转化生长因子Β1 氧化应激 

分 类 号：R285.5[医药卫生—中药学]