富硒大豆多肽对高脂致大鼠脂肪肝和抗氧化功能的影响  

Effects of Se-riched soybean peptide on antioxidant function in rats of fatty liver caused by high-fat diet

作　　者：王凤杰[1,2] 陈显兵[1,3] 张书毓[3] 谭志鑫[3] 向国敏[3] 刘锦红[3] 

机构地区：[1]湖北民族学院生物资源保护与利用湖北省重点实验室,恩施445000 [2]湖北民族学院科技学院,恩施445000 [3]湖北民族学院医学院,恩施445000

出　　处：《中国应用生理学杂志》2014年第4期339-342,I0002,共5页Chinese Journal of Applied Physiology

基　　金：生物资源保护与利用湖北省重点实验室项目(PKLHB1308);湖北民族学院科技学院科研项目(KY201410)

摘　　要：目的:探讨富硒大豆多肽改善高脂所致脂肪肝大鼠肝抗氧化功能的影响及机制。方法:将40只Wistar大鼠分成4组(n=10),分别饲喂标准饲料+水(NC)、标准饲料+富硒大豆多肽液(SeN)、高脂饲料+水(HC)、高脂饲料+富硒大豆多肽液(SeH),10周后处死,用苏丹Ⅲ染色肝脏组织切片观察脂肪变性程度,免疫组织化学方法测定肝组织葡萄糖调节蛋白78(GRP78)表达情况,并分析其肝功能、血脂及血清和肝匀浆中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的变化情况。结果:HC组在血清TC、TG水平、肝脏脂肪化程度、GRP78表达情况都明显高于NC、SeN、SeH组(P<0.01)。SeH组血清和肝组织中MDA含量较HC组降低(P<0.01),GSH-Px、SOD活性升高。NC和SeN两组各项指标之间无明显差异。结论:富硒大豆多肽能有效提高脂肪肝大鼠肝内抗氧化酶活性,抑制脂质过氧化反应,降低肝组织内GRP78表达。Objective： To explore the effect of Se-riched soybean pepfide（SSP） on ard.ioxidant function in rats of fatty liver caused by high-fat diet. Methods： Forty Wistar rats were divided into 4 groups randomly and fed with standard diet and water （NC）, high-fat diet and water （HC）, high-fat diet and SSP（ 0.1 g/d） （ Sell ）, standard diet and SSP（0.1 g/d ） （ SeN ） respectively. After 10 weeks, the rats were killed to investigate the pimelosis level in liver tissues by Sudan Ⅲ staining and the expression of hepatic GRP78 by anal- y-sls. We also analyzed the changes of liver function, blood lipid, the glutathione peroxidase（GSH-Px）, superoxide dismutase （SOD） activity and malondialdehyde （MDA） content in livers and serum. Results： The pimelosis level, total cholesterol （TC）, triglyceride （TG）, MDA contents and the expression of GRP78 in HC group were significantly higher than those in NC,SeN,SeH groups. The activities of GSH-Px and SOD in liver and serum were markedly up-regtdated in Sell （ P 〈 0.01）. There was no significant difference between NC and SeN groups. Conclusion： SSP can improve liver cell injury and the antioxidant functions in rats with fatty liver effectively and decrease the expression of GRP78 in liver.

关 键 词：富硒大豆多肽 脂肪肝 葡萄糖调节蛋白78 

分 类 号：R285.5[医药卫生—中药学]