E4031对慢性心衰大鼠离体心脏功能和心肌细胞内静息Ca^(2+)水平的影响  

Effects of E 4031 on isolated cardiac function and resting Ca^(2+) level of myocardial cells from rats with chronic heart failure

作　　者：白晓洁[1] 郝峻涛[2] 封启龙[1] 

机构地区：[1]山西医科大学生理学系细胞生理学省部共建教育部重点实验室,太原030001 [2]山西省人民医院胸外科,太原030012

出　　处：《中国应用生理学杂志》2014年第4期364-367,共4页Chinese Journal of Applied Physiology

摘　　要：目的:研究具有钠钙交换(NCX)激动作用的药物E 4031对慢性心衰大鼠离体心脏功能和心肌细胞内静息Ca2+水平的影响。方法:通过腹主动脉缩窄建立大鼠慢性心力衰竭模型;利用Langendorff装置进行离体心脏灌流,检测大鼠心功能及E 4031对血流动力学指标的影响;急性分离心衰大鼠心肌细胞,与钙荧光指示剂fluo3/AM共同孵育后,用激光共聚焦显微镜系统观察E 4031对心肌细胞内荧光强度的影响。结果:缩窄大鼠腹主动脉12周后,langendorff离体灌流检测显示大鼠心功能明显降低;在灌流液中加入10μmol/L E 4031可以使心衰大鼠心脏左室发展压(LVDP)和左室收缩/舒张最大速率(±dp/dtmax)提高;与正常组和伪手术组相比,心衰大鼠心肌细胞内静息钙荧光强度明显升高,和10μmol/L E 4031共孵育后,心衰大鼠心肌细胞静息钙荧光强度呈现短期先升后降过程,然后在较低的水平保持稳定。结论:E 4031可以增强慢性心衰大鼠离体心功能,可能与其增强心肌细胞膜NCX活动,稳定细胞内Ca2+水平有关。Objective： To investigate the effects of E 4031, a sodium calcium exehanger（NCX） agonist, on the isolated cardiac function and resting Ca^2＋ level in myocardial cells from rats with chronic heart failure. Methods： Rats chronic heart failure model was established by abdominal aorta coarctation with; Isolated heart perfnsion by Langendorff apparatus was used to detect heart function and the effects of E 4031 on haemedynamic indexes; Myocardial cells of rats in the model group were extracted quickly and coincubated with calcium fluorescent indicator fluo3/AM and the impact of E 4031 on the fluorescence intensity in myocardial cells were evaluated by confocal microscopy. Results： Heart function of rats in the model group detected by Langendorff peffusion was significantly reduced after 12 weeks, E 4031 at 10 μmol/L could improve their left ventricular developed pressure（LVDP） and systolic / diastolic maximum rate （ ＋ dp/dtmax）. Compared with the control and sham operation groups, the resting Ca^2＋ fluorescence intensity of the myocardial cells of rats in model group was at a higher level and went through a process of transient rise and drop, then stably remaining at a low level after co-incubated with 10 μmol/L E 4031. Conclusion： E 4031 can improve the isolated heart function of rats with chronic heart failure, which may be associated with its enhancing the activity of NCX in the myocardial cell membrane and stabilizing intracellular Ca^2＋ level.

关 键 词：E 4031 钠钙交换体 慢性心力衰竭 钙 心功能 

分 类 号：R331.3[医药卫生—人体生理学]