美容杜鹃叶片再生及愈伤组织耐热生理研究  

Study on Plantlet Regeneration for Blade Segments and the Physiological Trait for Heat Tolerance in the Callus of Rhododendron calophytum

作　　者：罗琳[1] 白洁[1] 陈超[1] 陈霞连 陈可[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《西北植物学报》2014年第7期1377-1382,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：四川省科技厅应用基础项目(2012JY0090);四川省科技基础条件平台项目-四川大学植物标本数字化平台建设及共享(2012JCP004)

摘　　要：以美容杜鹃幼叶为外植体,通过三因素三水平的正交试验,探究不同培养基、激素对愈伤组织诱导、分化和植株再生的影响,并对愈伤组织进行38℃热胁迫,探讨热胁迫下其耐热生理指标随时间的变化规律。结果表明:(1)愈伤组织诱导和芽分化最佳培养基均为Read+0.1mg/L NAA+0.2mg/L TDZ;最佳壮苗培养基为改良MS+0.1mg/L KT+0.2mg/L NAA;最佳生根培养基为1/2改良MS+2.0mg/L GA3。(2)随着热胁迫时间的延长,愈伤组织中的可溶性糖和游离脯氨酸含量变化不明显,而MDA和可溶性蛋白质的含量呈上升趋势,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性则表现为先升后降趋势;其中,MDA含量、可溶性蛋白质含量、SOD和POD活性随时间变化显著,可作为评价美容杜鹃耐热性的生理指标。The blade segments of Rhododendron calophytum were used as explants for the studying of callus induction and plantlets regeneration by orthogonal experimental design.Callus were kept at 38 ℃ for exploring physiological changes over time.The result revealed that：（1）Read＋0.1 mg/L NAA＋0.2 mg/L TDZ was the best medium for its callus induction and differentiation of adventitious bud,the improved MS ＋0.1 mg/L KT＋0.2 mg/L NAA was the better plantlet strengthening medium,and the 1/2 improved MS ＋2.0 mg/L GA3 was the optimum medium for rooting.（2）The contents of proline and soluble sugar in callus changed inconspicuously.MDA and solution protein contents were increased,and activities of SOD and POD were first increased and then decreased,which can be considered as the evaluation of heat resist ance system for R.calophytum.

关 键 词：美容杜鹃 叶片 愈伤组织 再生体系 耐热生理 

分 类 号：Q813.1[生物学—生物工程] Q945.78