人工合成甘蓝型油菜早期世代及其亲本对盐胁迫的生理响应  

Physiological Response in Early Generations of Resynthesized Brassica napus and Their Parents under Salt-stress

作　　者：杜坤[1] 孔芳[1,2] 孔月琴[1] 黄哲进 王幼平[1] 

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009 [2]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《西北植物学报》2014年第7期1383-1389,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国博士后科学基金(2013M541735);江苏省博士后科研资助计划(1301048B);江苏省高校自然科学基金(13KJB210007);江苏省研究生创新项目(CXZZ12_0897)

摘　　要：以白菜‘矮抗青’（基因组AA）和‘中花芥蓝’（基因组CC）及其人工合成异源四倍体甘蓝型油菜（AACC）的早期世代（F1 ～F4）为实验材料,采用水培方法分别比较它们在100、200 mmol/L NaC1处理下的生理指标差异.结果表明：（1）在盐胁迫条件下,‘中花芥蓝’植株的生物量、脯氨酸和叶绿素含量、抗氧化酶（SOD、POD、CAT）活性均最低,而相对电导率、MDA含量则最高.在100 mmol/L NaC1处理下,F2代植株的生物量、叶绿素含量、SOD活性最大,MDA含量最低;在200 mmol/L NaC1处理下,F4代的生物量、叶绿素含量、POD活性最大,MDA含量最低.研究发现,亲本‘矮抗青’的耐盐特性高于亲本‘中花芥蓝’,它们的杂种后代（异源四倍体）遗传了AA基因组的耐盐特性,从而比二倍体亲本具有更强的抵御盐胁迫的能力.In this study the difference of physiological characteristics of four early generations of synthesized Brassica napus allotetraploid（F1-F4） and their parents B.napus （AA）,B.oleracea （CC） under 100 mmol/ L,200 mmol/L NaC1 treatments through hydroponics culture was detected.The results demonstrated that the biomass,content of proline,relative chlorophyll,activities of antioxidant enzymes of B.oleracea were the lowest,while the relative conductivity,and MDA content were the highest.Furthermore,the biomass,relative chlorophyll content,activities of SOD of F2 generation were higher than other materials under 100 mmol/L NaC1 treatment,MDA content contrarily.The biomass,relative chlorophyll content,activities of POD of F4 generation were higher than others under 200 mmol/L NaC1 treatment,MDA content contrarily.The results of comparative analysis in the effect of varieties and salt stress suggested that B.oleracea with CC genome is more sensitive to salt stress than B.napus with AA genome.The salt tolerance of four synthesized Brassica early generations is better than diploid parents,because they inherit the salt tolerance performance of B.napus AA genome.

关 键 词：人工合成甘蓝型油菜 杂交后代 盐胁迫 生理指标 

分 类 号：Q945.78[生物学—植物学]