高频超声评价醛固酮在心肌梗死中致心肌损伤的作用  

Evaluation of the impact of aldosterone in cardiac remodeling after myocardial infarction by high-frequency ultrasound

作　　者：朱烨[1] 陈明[1] 张宇辉[1] 刘怡[1] 夏良华[1] 徐旻[1] 

机构地区：[1]上海,同济大学附属东方医院心脏医学部200120

出　　处：《中华超声影像学杂志》2014年第7期618-622,共5页Chinese Journal of Ultrasonography

基　　金：上海市浦东新区科技发展基金创新资金项目（PKJ2010-Y16）;上海市浦东新区心血管疾病学科群项目（PWZxkq2010-01）

摘　　要：目的应用小动物高频超声心动图评价醛固酮在急性心肌梗死后心肌损伤及重构中的作用。方法选取20只健康野生型小鼠作为对照组，20只醛固酮沉默小鼠作为实验组。采用左前降支结扎法建立心肌梗死动物模型。术前和术后2周、4周、6周分别利用小动物高频超声心动图测定各组小鼠的心功能指标，并于手术后6周处死实验组及对照组小鼠制做病理切片并行Masson染色。结果①高频超声心动图能清晰显示两组小鼠的左室长轴观及各短轴观图像，并可准确测量各项心功能指标。②冠状动脉左前降支结扎术后实验组小鼠的心脏收缩功能较对照组小鼠病情发展更缓和。结论阻止醛固酮在心肌中发挥作用对冠脉结扎术后的心脏收缩功能有积极作用。Objective To evaluate the value of high-frequency ultrasound in diagnosing the early reduced function of mice and to make sure the impact of aldosterone in cardiac remodeling after myocardial infarction. Methods Twenty aldosterone silence mice were selected as the experimental group and 20 healthy wild-type mice as the control.Animal models of acute myocardial infarction （AMI） were established by the method of the left anterior descending artery ligation. Cardiac function was measured by highfrequency echocardiography before and 2 weeks,4 weeks, 6 weeks after AMI. Produce biopsy and Masson staining in both the experimental and the control groups were made post 6 weeks after AMI.Results ①Left ventricular long axis view and various short-axis views of mice could be clearly displayed by high-frequency echocardiography,and accurate measurements of the cardiac function could be obtained. ②Compared with that in the control, left ventricular ejection fraction progressed more gradually in the experimental group than the control group （35.81±2.88 vs 29.10 ±4.50 at 6 weeks after AMI, P〈0.05）.Conclusions Blocked aldosterone may play a positive effect in reducing the myocardial injury.

关 键 词：超声心动描记术 心肌梗死 醛固酮 

分 类 号：R542.22[医药卫生—心血管疾病] R445.1[医药卫生—内科学]