脑血管痉挛的差异蛋白质组学研究  

Differential proteomics research of cerebral vasospasm

作　　者：甘渭河[1] 汤树洪[1] 梁敏[1] 梁道桥 冼克聪[1] 郑惠娜[1] 付强[2] 

机构地区：[1]贵港市人民医院神经外二科,广西贵港537100 [2]广西大学蛋白质组学实验室,广西南宁530004

出　　处：《中华神经外科疾病研究杂志》2014年第4期292-295,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：广西自然科学基金资助项目(2011GXNSFA018306)

摘　　要：目的采用蛋白质组学技术分离和鉴定动脉瘤性蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)患者组及无痉挛患者组发病后第1 d脑脊液中差异表达蛋白质,寻找与脑血管痉挛相关的蛋白质。方法采用双向凝胶电泳分离无脑血管痉挛组(对照组)、轻中度痉挛组(实验组1)和重度痉挛组(实验组2)脑脊液总蛋白质,比较分析实验组和对照组之间的差异表达蛋白质,应用基质辅助激光解析电离串联飞行时间质谱(MALDI-TOF/TOF-MS)鉴定差异表达的蛋白质。结果实验组1、实验组2与对照组表达差异2倍以上的蛋白质斑点共有68个,成功鉴定出23个差异表达蛋白质。结论初步建立了脑血管痉挛的差异表达蛋白质双向电泳技术体系,筛选出一批与血管痉挛相关蛋白质候选物。本研究为脑血管痉挛的发病机制研究提供了新的方法,为寻找脑血管痉挛的生物标志物、寻找药物治疗靶标和预后评估打下研究基础。Objective Proteomics technology is utihzed to isolate and identify differentially expressed proteins in cerebrospinal fluids within the first day after onset between patients with cerebral vasospasm （CVS） and without cerebral vasospasm after aneurysmal subarachnoid hemorrhage （SAH）. The study aims to find the proteins which are related to CVS. Methods Two-dimensional gel electrophoresis was used to separate the total proteins of cerebrospinal fluids in no vasospasm group （ control group）, mild-to-moderate vasospasm group （experiment group 1 ） and severe vasospasm group （experiment group 2）. Differential expressed protein spots were compared by image analysis of experiment groups and control group. MALDI-TOF/ TOF-MS was used to identify differentially expressed proteins. Results A total of 68 differential protein spots were characterized among experiment group 1, experiment group 2 and control group. A total of 23 differentially expressed proteins were identified successfully. Conclusion 2-DE technique of the differentially expressed protein profiling of cerebral vasospasm is initially established. Some proteins which may be related with cerebral vasospasm are screened. This study provides a new approach to study the pathogenesis of cerebral vasospasm. The research results lay a foundation for biomarkers finding, therapeutic target discovery and the prognosis evaluation of cerebral vasospasm.

关 键 词：双向凝胶电泳 蛋白质组学 基质辅助激光解析电离串联飞行时间质谱 颅内动脉瘤 脑血管痉挛 

分 类 号：R651[医药卫生—外科学]