PPAR-γ在液压脑损伤大鼠皮层的表达  

Expression of PPAR-γ in the cortex of the fluid percussion injury rats

作　　者：傅西安[1] 高国一[2] 蒲军[3] 冯军峰[2] 张夔鸣[2] 江基尧[2] 徐蔚[3] 

机构地区：[1]南京医科大学附属苏州医院神经外科苏州市卒中中心,江苏苏州215008 [2]上海交通大学附属仁济医院神经外科,上海200127 [3]昆明医科大学第二附属医院神经外科,云南昆明650101

出　　处：《中华神经外科疾病研究杂志》2014年第4期326-328,共3页Chinese Journal of Neurosurgical Disease Research

基　　金：南京医科大学科技发展基金面上项目资助(09NJMUM136);苏州市青年基金资助项目(SWKQ0820);苏州市科技发展计划基金资助(SZD09133)

摘　　要：目的通过随机分组研究侧方液压冲击脑损伤大鼠皮层过氧化物酶体增殖物激活受体-γ(peroxisome proliferator2 activated receptor gamma,PPAR-γ)信使核糖核酸(messenger ribonucleic acid,mRNA)的早期表达变化。方法雄性SD大鼠12只,随机分为两组:对照组(n=6);液压冲击脑损伤组(n=6),建立侧方液压冲击脑损伤大鼠模型,3 h后通过实时聚合酶链反应(real-time polymerase chain reaction,Real-time PCR)法检测伤侧皮层PPAR-γmRNA变化。结果侧方液压打击组与对照组比较,PPAR-γmRNA相对表达量明显降低(P<0.05)。结论抑制PPAR-γ的表达,促进炎性因子的释放可能为脑损伤机制之一。Objective The early expression of messenger ribonucleic acid （mRNA） of peroxisome proliferator2 activated receptor gamma （PPAR--γ ,） in the cortex of the fluid percussion injury rats is investigated. Methods A total of 12 male Sprague-Dawley （SD） rats were divided into 2 groups randomly, the control group （n = 6） and the fluid percussion injury group （ n = 6）. The lateral fluid percussion animal models were established to detect the changes of PPAR--γ , mRNA in the cortex by Real-time PCR 3 h after injury. Results Compared with the control group, the expression of PPAR--γ mRNA was decreased obviously in the fluid percussion injury group （P 〈0.05）. Conclusion Inhibition of PPAR--γ , expression and promotion of the releasing of inflammatory factors may be one of the mechanisms of brain injury.

关 键 词：创伤性脑损伤 过氧化物酶体增殖物激活受体-Γ 实时聚合酶链反应 

分 类 号：R651[医药卫生—外科学]