过敏性哮喘小鼠树突状细胞负荷Der p2抗原后表面标志物表达与细胞因子分泌水平的研究  

Effects of Der p2 on Surface Marker Expression and Cytokine Secretion of Dendritic Cells in Allergic Asthma Rats

作　　者：孔存权[1,2] 连利霞[1,2] 彭霞[3] 朱伟彦[1,2] 安云霞[1,2] 李莉[3] 

机构地区：[1]河南省人民医院 [2]郑州大学人民医院,河南郑州450003 [3]上海交通大学第一人民医院,上海200080

出　　处：《中国呼吸与危重监护杂志》2014年第4期398-401,共4页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：国家自然科学基金(编号:30671919;309728248)

摘　　要：目的探讨哮喘小鼠与正常小鼠骨髓源性树突状细胞负荷Der p2抗原后33D1、CD11c、CD86的表达及IL-10、IL-12p70分泌水平的差异,研究过敏性哮喘发病机制中树突状细胞的作用。方法分别从哮喘组和对照组小鼠提取骨髓培养树突状细胞,第7 d负荷Der p2抗原,24 h后观察树突状细胞形态,用流式细胞仪检测孵育后细胞表面33D1、CD11c、CD86的表达。并留取负荷Der p2前后培养上清液,ELISA法检测IL-10及IL-12p70含量。结果负荷Der p2抗原后,哮喘组CD86、CD11c表达较对照组显著升高[(54.02±5.32)%比(26.79±5.21)%,(67.51±5.99)%比(51.49±7.72)%,P<0.05];两组树突状细胞均表达小鼠树突状细胞特异性标志33D1,但表达水平均不高。在Der p2负荷前两组突状细胞均能分泌IL-10和IL-12p70,哮喘组IL-10水平高于对照组(P<0.05),IL-12p70水平低于对照组(P<0.05);在负荷Der p2后,对照组IL-10、IL-12p70分泌量较负荷前明显增加(P<0.05),而哮喘组负荷前后无明显变化。结论树突状细胞在过敏性哮喘中起着重要作用,异常树突状细胞通过增加CD86、CD11c的表达和减少IL-10及IL-12的合成,致使T细胞向Th2细胞优势分化。Objective To investigate the differences of 33D1, CDllc,CD86 expression and IL-10, IL-12p70 secretion from the dendritic cells （DCs） from allergic asthmatics and healthy donor rats after loaded with the Der p2 antigen, and to explore the possible role of DCs in the pathogenesis of allergic asthma. Methods Marrow-derived DCs were extracted and cultured, then collected after the Der p2 antigen loading 24 hours on the 7＇h day. The ceils morphology was observed,and the 33D1 ,CD11c ,CD86 expressions were measured with flow cytometry. Before and after the Der p2 loading, the culture supernatant was collected to measure the IL-10 and IL-12pT0 levels by ELISA. Results After loading with Der p2, compared with the control group, the expressions of CD86 and CDllc in the asthma group increased significantly [（54.02±5.32）% vs. （26.79±5.21）%,（67.51 ±5.99）% vs. （51.49 ±7.72）%,P〈 0.05 ] while the expression of DC -specific marker 33D1 was low in both groups. Both groups could secrete IL-10 and IL-12pT0 before Der p2 loading,with IL-10 higher in the asthma group and IL-12p70 higher in the control group （P 〈 0. 05 ）. After Der p2 loading, the levels of IL-10 and IL-12p70 increased significantly in the control group（P 〈0. 05 ）, and did not change obviously in the asthma group（ P 〉 0.05 ）. Conclusion These results identify that abnormal DCs with CD86 and CD11c over-expression can suppress the synthesis of IL-10 and IL-12 which may facilitate T cells differentiation into Th2 cells.

关 键 词：过敏性哮喘 树突状细胞 细胞因子 

分 类 号：R562.25[医药卫生—呼吸系统]