不同蛋白培养基对红色毛癣菌生长形态的影响  

The influence of culture media with different nitrogen and carbon nutrient sources on the biological characters of Trichophyton rubrum

作　　者：胡婵[1,2,3] 朱红梅[2,3] 谭宏月[1] 李平[2,3] 宋维芳[4,5] 顾军[1] 温海[2,3] 

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433 [2]上海长征医院皮肤病与真菌病研究所全军真菌病重点实验室 [3]第二军医大学附属长征医院皮肤科,上海200003 [4]厦门大学附属成功医院 [5]解放军第174医院,厦门361003

出　　处：《中国真菌学杂志》2014年第3期139-142,146,共5页Chinese Journal of Mycology

基　　金：解放军南京军区医学科技创新课题(09MA065);军队十二五课题(BWS12J028)

摘　　要：目的观察不同蛋白及碳源培养基对红色毛癣菌生物学特性的影响。方法将红色毛癣菌以点接种方式分别接种于马铃薯葡萄糖琼脂培养基、胰蛋白琼脂培养基、角蛋白葡萄糖培养基、葡萄糖琼脂培养基上,培养2周后观察菌落形态,拍照及测量菌落面积;比较观察不同浓度角蛋白对菌生长形态的影响;比较观察葡萄糖对红色毛癣菌生长形态的影响。结果上述蛋白质作为氮源均可促进红色毛癣菌生长。其中在角蛋白生长更加良好,并产生最明显的红色色素;葡萄糖作为碳源能够有效促进红色毛癣菌的生长,在角蛋白培养基中能够促进红色色素的生成。结论培养基中角蛋白含量影响红色毛癣菌的生长状况,葡萄糖是决定红色毛癣菌红色素量的决定性因素。本研究有助于整体认识红色毛癣菌的生长代谢机制,通过表型的研究为进一步揭示相关基因的差异性表达提供基础,为防治红色毛癣菌引起的癣病及抗真菌药物的开发提供策略相关的基础研究。Objective To find the influence of culture medium with different kinds of nitrogen and carbon source on the growth morphology of Trichophyton rubrum.Method Point inoculated the Trichophyton rubrum on potato dextrose medium,trypsin agar medium,keratin glucose medium and glucose agar medium.Observed the morphology and tested the area of the colony after 2 weeks of incubation.Compared the growth morphology of Trichophyton rubrum when inoculated with and without glucose.Compared the influence of different keratin concentration to the growth morphology of Trichophyton rubrum.Result Different protein as nithogen source in different medium could all promote the growth of Trichophyton rubrum.Yet Trichophyton rubrum growed better in keratin culture and produced more red pigment.Glucose added to the keratin culture as carbon source could effectively promote the growth of Trichophyton rubrum and the production of red pigment.Conclusion The concentration of keratin in the cuhure medium can affect the growth of Trichophyton rubrum,and the glucose is the key factor to the production of red-pigment for Trichophyton rubrum.The overall understanding of metabolic mechanism of Trichophyton rubrum might provide a good basis for further study and even a good strategy of the antifungal research.

关 键 词：红色毛癣菌 角蛋白 葡萄糖 红色色素 

分 类 号：R379.2[医药卫生—病原生物学]