清醒状态下热射病大鼠胃肠动力与胃泌素、胃动素的变化  

Gastrointestinal motility and changes of serum motilin and gastrin in conscious heat stroke rats

作　　者：周秋娟[1] 聂占国[2] 代忠明[2] 张新[2] 董翔[3] 

机构地区：[1]石河子大学医学院,新疆维吾尔自治区石河子市832000 [2]兰州军区乌鲁木齐总医院消化科,新疆维吾尔自治区乌鲁木齐市830000 [3]兰州军区乌鲁木齐总医院实验动物科,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《世界华人消化杂志》2014年第21期3003-3010,共8页World Chinese Journal of Digestology

基　　金：兰州军区课题基金资助项目;No.CLZ12JB17~~

摘　　要：目的:通过观察清醒状态热射病(conscious heat stroke,CHS)大鼠胃肠黏膜腺体形态学改变,探讨其胃肠动力与血浆胃泌素(gastrin,GAS)、胃动素(motilin,MTL)含量的变化.方法:首先使用人工实验环境模拟舱模拟清醒状态热射病大鼠模型,光镜下观察胃肠黏膜腺体的形态学改变;胃肠内标志物法测定大鼠胃排空率(gastric emptying rates,GERS)、小肠推进率(small intestinal propulsion rates,SIPRS),使用ELISA法检测清醒状态热射病(conscious heat stroke,CHS)大鼠血液和胃肠组织中GAS、MTL含量;应用免疫组织化学技术观察胃肠黏膜腺细胞GAS、MTL的表达情况.结果:(1)与正常对照组相比,模型组GERS、SIPRS明显降低(P<0.05),大鼠血清GAS、MTL显著升高(P<0.05),16 h时达峰值后迅速下降;而胃组织GAS峰值前移至8 h,十二指肠组织MTL随致热时间延长明显降低(P<0.05);(2)形态学方面:与正常大鼠相比,模型组大鼠随致热时间延长,大鼠胃肠黏膜腺体结构紊,腺上皮排列不规整、浊肿、脱落、腺腔狭小或缺失,腺体结构消失,腺体胞浆内褐色颗粒表达物由深染、大小不一、分布不均匀转化为量少、淡染.结论:血中MTL、GAS含量变化测定既可作为判断CHS大鼠胃肠动力障碍的指标,亦可作为判断大鼠热射病发生、发展的指标.AIM： To establish a conscious heat stroke （HS） rat model to investigate heat stroke-induced gastrointestinal motility and changes of serum motilin （MTL） and gastrin （GAS）.METHODS： A conscious HS rat model was established using artificial experimental environ- ment simulation cabin （AEESC）. The changes of gastric mucosal gland morphology was observed by light microscopy. The blue Sephadex-2000 marker was used to determine the rates of gastric emptying （GAS） and small intestinal transit （SIT）. Serum and gastrointestinal contents of MTL and GAS were detected by ELISA, and expression of GAS and MT in the gastrointestinal mucosa gland was observed by the immunohis- tochemical technique. RESULTS： Compared with normal controls, the rates of GE and SIP were significantly lower in HS rats, while serum GAS and MTL levels were significantly higher （P 〈 0.05）. Serum GAS and MTL peaked at 16 h and then rapidly decreased, while gastric GAS peaked at 8 h. The longer the thermal stimulation time, the lower the concentration of MTL in the duodenum （P 〈 0.05）. With the increase of thermal stimulation time, the rats showed gastric mucosal gland structure disturbance, glandular epithelial disarrangement, cloudy swelling, and glandular cavity narrowing or missing. Finally, glandular epithelial degeneration and gland structure destruction were observed, and the size, color and distribution of granules in the cytoplasm of gland cells were significantly changed. CONCLUSION： The changes of serum MTL and GAS may be indicators to judge abnormal gastro- intestinal motility in conscious heat stroke rats.

关 键 词：热射病 胃肠动力 胃动素 胃泌素 

分 类 号：R594.1[医药卫生—内科学]