半胱氨酰白三烯受体-1在大鼠肠缺血再灌注损伤中的作用  

Role of cysteinyl leukotriene receptor 1 in intestinal ischemia-reperfusion injury

作　　者：吴深宝[1] 朱旭星[1] 金忠海[1] 童秀萍[1] 洪小飞[1] 沈卫东[2] 

机构地区：[1]温州医学院附属义乌中心医院消化科,浙江省义乌市322000 [2]南通大学附属江阴医院消化科,江苏省江阴市214400

出　　处：《世界华人消化杂志》2014年第21期3089-3094,共6页World Chinese Journal of Digestology

基　　金：浙江省医学会临床科研基金资助项目;No.2012ZYC-A87~~

摘　　要：目的:探讨半胱氨酰白三烯受体1(cysteinyl leukotrienes receptor,CysLTsR1)在肠缺血再灌注(intestinal ischemia-reperfusion,I/R)损伤中的可能作用.方法:以I/R大鼠肠I/R损伤模型为研究对象,并运用不同剂量CysLTsR1拮抗剂孟鲁司特(0.2、2和20 mg/kg)加以干预,通过免疫组织化学染色方法和RT-PCR法检测大鼠肠组织CysLTsR1蛋白和mRNA表达水平,并应用光学显微镜观察小肠病理改变、检测小肠质量变化、确定CysLTsR1拮抗剂孟鲁司特对小肠的炎症程度的影响.结果:肠I/R可造成肠组织损伤,小肠含水量增加(模型组78.13 g±1.35 g vs正常组78.13 g±1.35 g,P<0.05),肠I/R时肠组织CysLTsR1蛋白和mRNA表达水平升高(P<0.05);应用孟鲁司特后,小肠含水量降低(低、中、高剂量组分别为76.47 g±1.48 g、72.67 g±5.35 g及75.39 g±3.66 g),肠组织损伤减轻,肠组织CysLTsR1蛋白表达和mRNA表达水平均减低(P<0.05).结论:CysLTsR1参与了肠I/R损伤的形成,孟鲁司特可以减轻肠I/R对肠组织的损伤,CysLTsR1是保护肠I/R损伤的新的作用靶点.AIM： To explore the role of cysteinyl leukotriene receptor 1 （CysLTsR1） in intestinal ischemia- reperfusion （I/R） injury. METHODS： Forty rats were divided into 5 groups： a control group, a model group and low-, medium- and high-dose montelukast groups. After treatment, the water content of the small bowel was determined, and the his- tological changes in the intestinal tissues were assessed by HE staining. Immunohistochemical assessment of the expression of CysLTR1 and 5-1ipoxygenase （5-LOX） was also performed.RESULTS： Intestinal I/R, small bowel water content （model group 78.13 g ± 1.35 g vs normal group 78.13 g ± 1.35 g, P 〈 0.05）, and CysLTR1 protein and mRNA expression significantly in- creased in the model group compared with the control group. Application of montelukast de- creased intestinal water content （low-, medium-, and high-dose groups 76.47 g ± 1.48 g, 72.67 g ± 5.35 g and 75.39 g ± 3.66 g vs model group 78.13 g± 1.35 g, P 〈 0.05）, relieved the intestinal tissue injury, and reduced CysLTR1 protein and mRNA expression in intestinal tissues （P 〈 0.05）. CONCLUSION： CysLTR1 participates in the pathogenesis of intestinal ischemia-reperfusion injury, and may be a target for treatment of I/R injury.

关 键 词：半胱氨酰白三烯受体1 肠缺血再灌注 孟鲁司特 

分 类 号：R574[医药卫生—消化系统]