海南龙血树查尔酮合酶基因(DcCHS)的克隆及表达分析  

Cloning and Expression Analysis of Chalcone Synthase Gene(DcCHS) in Dracaena cambodiana Pierre ex Gagnep

作　　者：王佳媛[1,2] 戴好富[2] 李辉亮[2] 郭冬[2] 彭世清[2] 梅文莉[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2014年第8期1539-1545,共7页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31070321);公益性行业(农业)科研专项(No.201303117);海南省重大科技专项(No.ZDZX2013023-1;No.ZDZX2013008-4)

摘　　要：利用RT-PCR和RACE技术对海南龙血树查尔酮合酶基因进行克隆,得到1条1 456 bp的cDNA序列,命名为DcCHS。DcCHS含有1 173 bp的阅读框架、99 bp的5′非编码区和184 bp的3′非编码区,编码390个氨基酸。DcCHS与其他植物的CHS氨基酸序列同源性高达84%以上,具有高度保守的CHS_like结构域、活性位点以及信号序列。推测DcCHS的分子量为42.7 ku,等电点pI为6.14,稳定性极差,具有15个磷酸化位点,无跨膜结构,无信号肽,亚细胞定位在细胞质的可能性较大,并预测了蛋白质的二级、三级结构。组织特异性分析结果表明,DcCHS在花中的表达远远高于根、茎、叶和果实。The full-length cDNA encoding chalcone synthase, designated as DcCHS, was isolated from Dracaena cambodiana by the RACE method. DcCHS contained a 1 173 bp open reading frame with 99 bp 5＇UTR and 184 bp 3＇UTR. The deduced DcCHS protein consisted of 390 amino acid residues with a calculated molecular weight of 42.7 ku and isoelectric point （pI） of 6.14. The deduced DcCHS, which shared more than 84% identities with the CHS protein from other plants, was predicted to possess the conserved CHS_like domain, active sites and signal sequences. DcCHS expressed at different levels with the highest transcription in the flowers, and the lowest transcription occurring in stems. The present study perhaps contributes towards an understanding of the characteristics in expression mechanism of DcCHS.

关 键 词：海南龙血树 查尔酮合酶 克隆 表达 

分 类 号：Q78[生物学—分子生物学]