PEG胁迫对2种蕨类原叶体抗旱生理特性的影响  

Effect of PEG Stress on Drought Resistance Physiological Indices of Two Ferns' Prothallus

作　　者：李巧娟[1] 钟倩云[1] 邵莉[1,2] 季华[1] 陈龙清[1] 

机构地区：[1]园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院,武汉430070 [2]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《中国农学通报》2014年第22期155-160,共6页Chinese Agricultural Science Bulletin

基　　金：中央高校基本科研业务费专项基金资金(2013PY089)

摘　　要：研究不同浓度的聚乙二醇(PEG-6000)渗透胁迫下荷叶铁线蕨和扇叶铁线蕨原叶体的生理指标的变化。采用人工模拟干旱胁迫的方法,选取3种浓度(0%、15%、30%)进行胁迫处理,研究了干旱胁迫对其细胞形态结构、渗透调节物质和保护酶活性的影响。随着胁迫程度的加深,原叶体颜色越暗且长势变差;处理组(15%和30%)的丙二醛(MDA)含量均低于对照组(0%);游离脯氨酸(Pro)的含量在胁迫期间增加不明显;可溶性蛋白(SP)含量均先升后降,都较对照的高,差异显著;荷叶铁线蕨原叶体不同处理的超氧物歧化酶(SOD)活性均呈先升后降趋势,而扇叶铁线蕨的轻度胁迫(15%)和重度胁迫(30%)都下降;过氧化氢酶(CAT)活性随着干旱程度的增加均显著上升。该研究说明SP和CAT活性可作为评价这2种蕨类原叶体抗旱性的重要指标,可为蕨类植物的开发利用提供理论依据。Different concentrations of PEG were used to assess the effect on morphological characteristics and physiological indices of the prothallus of Adiantum reniforme var. sinense and Adiantum flabellulatum. By means of artificial simulated drought stress, three concentrations （0%, 15%, 30%） of PEG were selected to study the change of cell morphology, protective enzyme activity and osmoregulatory molecules in leaves. With drought stress deepening, the prothallus became darker and grew worse; MDA content of two treatment groups （15% and 30%） was lower than that of the control group; Pro content did not increase distinctly during the period of drought stress; SP content of two treatment groups all increased firstly and then decreased, and they were remarkably higher than that of the control group; SOD activity of three groups of Adiantum reniforme var. sinense increased first and then decreased, while two treatment groups of Adiantumflabellulatum decreased all the time; CAT activity was increasing significantly. It was concluded that SP and CAT activity could reflect the ability of drought resistance for these two ferns＇ prothallus. The study could also provide theoretical basis for the development and utilization of fern.

关 键 词：干旱胁迫 荷叶铁线蕨 扇叶铁线蕨 形态特征 生理响应 

分 类 号：S682.35[农业科学—观赏园艺]