洛阳市番茄灰霉病菌对嘧霉胺的抗药性检测  

Detection of the Resistance of Botrytis cinerea to Pyrimethanil from Tomato in Luoyang

作　　者：刘圣明[1] 张艳慧[1] 高续恒 李梦[1] 梅笑寒 

机构地区：[1]河南科技大学林学院植物保护系,河南洛阳471003

出　　处：《中国农学通报》2014年第22期300-303,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目"抗嘧霉胺的番茄灰霉病菌中CGS和MS基因的功能研究"(31301688);公益性行业(农业)科研专项"农作物重要病原菌抗药性监测及治理技术研究与示范"(201303023);河南省教育厅科学技术研究重点项目"设施蔬菜灰霉病抗药性群体形成机制及其综合治理技术研究"(13A210277);河南科技大学博士科研启动基金项目"河南省农作物重要病原菌抗药性群体形成机制研究"((09001589)

摘　　要：为了明确洛阳市番茄灰霉病菌对苯胺基嘧啶类杀菌剂嘧霉胺的抗药性状况,2012—2013年从洛阳市不同保护地中采集番茄灰霉病病果或病叶,经单孢分离共获得番茄灰霉病菌54株。采用菌丝生长速率法测定了其对嘧霉胺的敏感性。结果显示,高抗菌株9株(16.67%),中抗菌株19株(35.19%),低抗菌株10株(18.52%),敏感菌株16株(29.63%)。比较抗性水平不同菌株间的菌丝生长情况,发现菌丝生长速度和抗性水平无相关性。研究表明,洛阳市番茄灰霉病菌已对嘧霉胺产生不同水平的抗药性,且以中抗菌株为主,生产上应合理更换或轮换使用与嘧霉胺无交互抗性的药剂防治番茄灰霉病。To investigate the resistance of Botrytis cinerea to pyrimethanil, fifty-four Botrytis cinerea strains were isolated from diseased tomato fruits or leaves collected in 2012 and 2013, from different regions of Luoyang, and evaluated by the method of hypha growth inhibition for the resistance to pyrimethanil. The results demonstrated that the frequency of high, middle, and low resistance and sensitivity were 16.67%, 35.19%, 18.52%, and 29.63%, respectively. There was no significant correlation between the mycelium growth and pyrimethanil-resistance or sensitivity. The research showed that the different levels resistance to pyrimethanil of Botrytis cinerea from tomato have been found, and middle-level resistance was common in Luoyang. The management tomato grey mould disease should be replaced or rotated using reasonable pyrimethanil no cross- resistance of fungicide.

关 键 词：灰霉病 灰葡萄孢菌 嘧霉胺 抗药性 

分 类 号：S481.4[农业科学—农药学]