神经调节蛋白-1干预对大鼠心肌梗死后低电压区起搏阈值的影响  

Influence of neuregulin-1 on pacing threshold of low voltage area in rats after myocardial infarction

作　　者：王召军[1] 曲秀芬[1] 李晶洁[1] 于彦伟[1] 孙琳[1] 曹威[1] 

机构地区：[1]哈尔滨医科大学附属第一医院心内科,黑龙江哈尔滨150001

出　　处：《心血管康复医学杂志》2014年第4期388-391,共4页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：黑龙江省卫生厅立项(2012-536);哈尔滨医科大学附属第一医院院基金支持(2011Y05)~~

摘　　要：目的:探讨神经调节蛋白-1(NRG-1)干预对大鼠心肌梗死后低电压区起搏阈值的影响。方法:采用冠状动脉前降支结扎法建立心肌梗死大鼠模型,34只心肌梗死大鼠被随机均分为两组:NRG-1干预组(接受NRG-1腹腔注射)和对照组(接受同体积生理盐水腹腔注射)。2周后测试大鼠心肌梗死后梗死相关的低电压区的起搏阈值,并检测低电压区Cx43(连接蛋白家族的一种蛋白质)的表达。结果:药物干预2周时,NRG-1组起搏阈值明显低于对照组起搏阈值[(0.7167±0.194)V比(1.466±0.503)V,P=0.002];心肌梗死后低电压区NRG-1组Cx43表达较对照组明显增多[(0.95±0.20)比(0.30±0.15),P<0.001]。结论:NRG-1明显降低大鼠心肌梗死后低电压区起搏阈值,其机制可能与增加Cx43表达,改善心肌细胞间电传导有关。Objective： To explore the influence of neuregulin- 1 （NRG-1） intervention on pacing threshold of low voltage area in rats after myocardial infarction. Methods： Myocardial infarction model of rat was established via an terior descending coronary artery ligation. The 34 rats with myocardial infarction were randomly and averagely divided into two groups. NRG-1 group （received NRG-1peritoneal injection） and control group （received peritoneal injection of normal saline of the same volume）. After two weeks, pacing thresholds were tested in low voltage area related with infarct in rats after myocardial infarction, and Cx43 （an integral membrane protein of the connexin family） expression was measured in low voltage area. Results.. After drug intervention two weeks, compared with control group, there was significant reduction in pacing threshold [ （1. 466±0. 503） V vs. （0. 7167 ±0. 194） V, P = 0. 0021 in NRG-1 group; there was significant rise in Cx43 expression [（0. 30 ± 0.15） vs. （0.95 ± 0.20）, P〈0. 001] in low voltage area in NRG-1 group. Conclusion： Neuregulin-1 significantly reduces pacing threshold of low voltage area in rats after myocardial infarction, the mechanism may be related to increase Cx43 expression and improve electrical conductivity of mvocardial cells.

关 键 词：神经调节蛋白1 心肌梗塞 大鼠 

分 类 号：R542.220.9[医药卫生—心血管疾病]