农杆菌介导的梭梭BADH基因转化玉米的研究  

Study on the Maize Transformation with Haloxylon ammodendron BADH Gene by Agrobacterimu tumefaciens

作　　者：母养秀[1,2] 甘晓燕[1] 聂峰杰[1] 宋玉霞[1] 

机构地区：[1]宁夏农业生物技术重点实验室,银川750002 [2]宁夏固原市农业科学研究所,宁夏固原756000

出　　处：《中国农学通报》2014年第24期299-302,共4页Chinese Agricultural Science Bulletin

基　　金：宁夏回族自治区科技支撑项目"抗旱;耐盐碱植物功能基因挖掘与利用研究"(2012ZYS041)

摘　　要：探究影响玉米遗传转化效率的因素,对玉米遗传转化系统的建立与完善有重要意义。以‘宁玉0905’为材料,经农杆菌介导将梭梭的BADH基因转入玉米基因组。结果表明:在黑暗条件下,加入乙酰丁香酮(AS),玉米遗传转化效率提高;当菌液浓度OD600=0.3,浸染时间为5 min时,玉米的转化效率最高;农杆菌和愈伤组织共培养2天,玉米的遗传转化效果最好。对转化苗进行PCR检测得出BADH基因已整合到玉米基因组中。当菌液的OD600=0.3,浸染时间为5 min,农杆菌和愈伤组织共培养2天时,玉米的转化效率最高。This study explored the effect of factors on Maize transformation efficiency and had importantsignificance to establish and perfect the maize genetic transformation system. Taking the‘NingYu 0905＇asthe study material, the transcription factor BADH gene was transformed into Maize genome of‘NingYu 0905＇by Agrobacterium tumefaciens. The results revealed that adding acetosyringone could improve thetransformation efficiency in corn in darkness. The highest transformation efficiency in corn was obtained when OD600 Agrobacterium tumefaciens was 0.3 for 5 min. The best transformation efficiency in corn wasAgrobacterium tumefaciens and callus in 2 d CO-cultivation. The BADH gene was proved to be transferred intomaize genome by PCR test of transformed plants. The highest transformation efficiency in corn was detected in2 dCO-cultivationafter 0.3of OD600 Agrobacterium tumefaciensfor 5 min.

关 键 词：玉米 遗传转化 农杆菌介导 BADH基因 

分 类 号：Q7[生物学—分子生物学]