聚丙烯酰胺降解菌筛选与分子生物学鉴定  

Isolation and molecular identification of polyacrylamide degrading bacteria

作　　者：任国领[1] 徐晶雪[1] 王晶[1] 张虹[1] 乐建君[2] 黄永红[1] 

机构地区：[1]大庆师范学院生命科学学院,黑龙江大庆163712 [2]大庆油田有限责任公司勘探开发研究院,黑龙江大庆163712

出　　处：《东北石油大学学报》2014年第4期67-71,9-10,共5页Journal of Northeast Petroleum University

基　　金：大庆师范学院博士启动基金项目(12ZR06);黑龙江省青年科学基金项目(QC2011094)

摘　　要：从大庆油田聚驱后油藏采出水中筛选出一株降解聚丙烯酰胺的细菌菌株——QSJU001.该菌株能在以聚丙烯酰胺为惟一碳源的无机盐培养基中生长.对该菌进行16SrDNA序列的分子生物学鉴定,测得的16SrDNA序列与GenBank中最相近的前10个菌株的相似性均为99%.利用该菌属与最相近的前10个菌株构建该菌种的系统发育树,结果显示该菌株与Acinetobacter baumannii strain KK14菌株的碱基差别最小.该菌的形态特征观察、生理生化特征检测、16SrDNA分子生物学鉴定结果表明,菌株QSJU001与Acinetobacter baumannii strain KK14亲缘关系最近,属于不动杆菌属.降解率实验结果表明,菌株QSJU001对聚丙烯酰胺降解率基本稳定在33%左右,对聚丙烯酰胺的降解效果较好.Abatrct:A microbial strain that could degrade polyacrylamide and grow on HPAM as the sole source of carbon was isolated from reservoirs after polymer flooding in Daqing oilfield.This strain was named as QSJU001.Appraised by the strain 16 SrDNA molecular biology,its alignment of 16 SrDNA sequence had 99%similarity with those of the first ten strains of nearest heredity distance in GeneBank.Using16 SrDNA sequence and the first ten strains of 16 SrDNA sequence to create a phylogenetic tree,wefound that nucleotide difference between QSJU001 and Acinetobacter baumannii strain KK14 is minimum.Morphological,physiological and biochemical,molecular biological identification results show QSJU001 is identified as Acinetobacter sp..The results of degradation rate experiment show that degradation rate for polyacrylamide of QSJU001 is around 33%.It has a good effect on the degradation for polyacrylamide.

关 键 词：聚丙烯酰胺 降解菌 筛选 不动杆菌属 

分 类 号：TE357.43[石油与天然气工程—油气田开发工程]