萘酰亚二胺-多胺缀合物与DNA作用的光谱研究  

Spectroscopic Study on the Interaction Between Naphthaliimide-Polyamine Conjugates and DNA

作　　者：田智勇[1] 王玉霞[1] 赵中华[1] 赵瑾[1,2] 王超杰[1,2] 

机构地区：[1]河南大学化学生物学研究所,河南开封475004 [2]河南大学天然药物与免疫工程重点实验室,河南开封475004

出　　处：《安徽师范大学学报（自然科学版）》2014年第4期357-362,共6页Journal of Anhui Normal University(Natural Science)

基　　金：河南省科技厅项目(132102310026;112300410181);河南省教育厅项目(14A350004)

摘　　要：在人体生理条件下(pH=7.4),采用紫外光谱法和荧光光谱法研究了萘酰亚二胺-多胺缀合物(1)与鲱鱼精DNA的作用.研究发现DNA的加入使化合物1的紫外吸收光谱发生减色效应且出现红移现象,提示化合物1与DNA发生强烈的嵌插作用.采用变温荧光光谱法研究发现荧光淬灭机制为静态淬灭,根据荧光数据计算出不同温度下的结合常数、结合位点数及热力学参数,发现化合物与DNA作用力类型主要是氢键.The interaction of naphthaliimide-polyamine conjugate with herring sperm DNA was studied by UV spectrum and fluorescence spectrum under physiological conditions （pH =7.4）.The hypochromicities and red shifts were observed from the absorption titration experiments.These results indicated that compound 1 intercalated into the DNA.Through the fluorescence quenching data measured at different temperatures （25,30and 37℃） revealed that the quenching mechanism was a static type.Meanwhile,the obtained binding constant and thermodynamic parameters on compound-DNA interaction showed that the type of interaction force of compound 1 and DNA was mainly hydrogen bond.

关 键 词：萘酰亚二胺 多胺缀合物 DNA 光谱法 

分 类 号：O657.3[理学—分析化学]