鲻鱼鳃组织14-3-3a、NKCCla、Apo-14和Na^+-K^+-ATPaseβ基因表达对盐度变化的响应  

Effects of abrupt salinity stress on gill 14-3-3a,NKCCla,Apo-14 and Na^+-K^+-ATPaseβ expression of Mugil cephalus

作　　者：蒋玫[1] 李磊[1] 沈新强[1] 吴庆元[1] 牛俊翔[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090

出　　处：《生态学杂志》2014年第9期2429-2435,共7页Chinese Journal of Ecology

基　　金：国家基础研究发展计划项目(G2010CB429005)资助

摘　　要：探讨了鲻鱼(Mugil cephalus)鳃组织14-3-3a、NKCCla、Apo-14和Na+-K+-ATPaseβ基因对盐度变化的响应表达特性。结果表明:不同盐度处理组对14-3-3a、NKCC1a、Apo-14和Na+-K+-ATPaseβ等4种基因mRNA的表达量与对照组(盐度20)间存在一定的差异性,且各类基因与盐度适应相关性的差异性也较为显著;其中Na+-K+-ATPaseβ和NKCC1a在低盐环境下被显著诱导,表达量上升明显(P<0.01),而14-3-3a与Apo-14在低盐环境下表达量均有明显的下调(P<0.05);不同盐度处理组与对照组表达量的差异、各基因表达量与盐度的回归关系以及4种基因生物整合标志物响应值的不同,表明Na+-K+-ATPaseβ和NKCC1a相对于14-3-3a和Apo-14更能赋予生物在受到低盐度应激刺激后,产生渗透调节机制,提高生物抵抗环境的胁迫能力;可考虑Na+-K+-ATPaseβ和NKCC1a适宜作为鱼类鳃组织在低盐环境胁迫下调控渗透基因的潜在分子生物标志物。To explore the response of gene expression of gill 14-3-3a, NKCCla, Apo-14 and Na＋- K＋-ATPaseβ in Mugil cephalus to salinity stress, polymerase chain reaction-restrained fragment length polymorphisms technique was applied. We found that NKCCla and Na＋-K＋-ATPaseβ pro- tein synthesis was induced at the low salinity stress, with an obvious increase in expression level （P〈0.01）. However, the 14-3-3 gene mRNA expression level and Apo-14 gene mRNA expression level descended at the low salinity stress （P〈0.05）. The comprehensive evaluation index of multiple biomarkers was established based on the integrated biomarker responses （IBR）. The IBR values of each gene indicated that the NKCCla and Na＋-K＋-ATPaseβ genes were suitable molecular biomarkers of the fish gill tissue for salinity stress assessment.

关 键 词：鲻鱼 14-3-3a NKCCla Apo-14 Na+-K+-ATPaseβ 基因表达 

分 类 号：S917.4[农业科学—水产科学]