结核病患者治疗过程中外周血B细胞及其亚群的变化  

Analysis of B cells and B cell subsets in the peripheral blood of patients with pulmonary tuberculosis in treatment process

作　　者：李美忠[1] 张影[1] 许颖琪[2] 张洁云[1] 张明霞[1] 陈心春[1] 邓群益[2] 

机构地区：[1]深圳市第三人民医院研究所,广东深圳518112 [2]深圳市第三人民医院结核病科,广东深圳518112

出　　处：《国际检验医学杂志》2014年第12期1552-1554,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金面上项目(81241065);深圳市科技计划项目(20120264)

摘　　要：目的在比较健康人、结核潜伏感染者、活动性肺结核患者B细胞及亚群变化的基础上了解活动性肺结核患者治疗过程中B细胞百分比及B细胞亚群的分布情况。方法根据B细胞表面CD19/CD5/CD1d标识将B细胞分为四群。利用流式细胞术比较活动性肺结核患者治疗前、治疗3个月、治疗6个月、治疗9个月时外周血B细胞百分比及B细胞分群表达的差异。结果结核患者治疗6个月时外周血B细胞较治疗3个月时有所增加(P<0.01)。结核患者治疗3个月时外周血中CD19+CD5-CD1d+和CD19+CD5+CD1d+B细胞百分比最高,并随治疗时间的延长而逐渐下降(P<0.01)。结核患者治疗3个月时CD19+CD5-CD1d-B细胞百分比较治疗前下降(P<0.01),但随治疗时间的延长而逐渐上升(P<0.01)。结核患者外周血CD19+CD5+CD1d-B细胞百分比在治疗后逐渐上升,结果有统计学差异(P<0.01)。结论肺结核患者外周血中紊乱的B细胞亚群在治疗过程中,逐渐恢复正常,提示B细胞亚群在结核的发病中起重要作用。Objective To explore the dynamic variation of B cell subsets in the patients with tuberculosis （TB）.Methods Ac-cording to expression of CD19/CD5/CD1d on the surface of cells,B cells were divided into four groups.B cell subsets in peripheral blood of TB patients at different stages were analyzed by using flow cytomety.Results The frequency of B cells in TB patients af-ter treatment of 6 months was higher than treatment of 3 months （P〈0.01）.Proportion of CD19＋CD5-CD1d＋B cells in TB pa-tients after treatment of 3 months was the highest,but gradually declined after treatment of 6 months and 9 months （P〈0.01）. Proportion of CD19＋CD5＋CD1d＋B cells in TB patients after treatment of 3 months was the highest,but gradually declined after treatment of 6 month and 9 months （P〈0.01）.Proportion of CD19＋CD5-CD1d-B cells in TB patients after treatment of 3 months was lower than before treatment （P〈0.01）,but gradually increased after treatment of 6 months and 9 months （P〈0.01）. Proportion of CD19＋CD5＋CD1d-B cells gradually increased after treatment （P〈0.01）.Conclusion B cell subsets might be con-fused in peripheral blood of TB patients,but could gradually returned to normal level after treatment.B cell subsets might play an important role in morbidity of TB.

关 键 词：结核 B淋巴细胞 细胞亚群 

分 类 号：R52[医药卫生—内科学]