恩替卡韦对慢性乙型肝炎患者HBV cccDNA水平的影响  

Influence of entecavir on HBV cccDNA levels in patients with chronic hepatitis B

作　　者：张宝[1] 李世龙[2] 朱敏[3] 秦莉[1] 李小林[1] 田珊红[1] 张翠华[1] 张燕[1] 

机构地区：[1]河北省唐山市传染病医院检验科,河北唐山063000 [2]河北省唐山市人民医院检验科,河北唐山063000 [3]国家安全生产监督管理总局职业安全卫生研究所石龙医院,北京102300

出　　处：《国际检验医学杂志》2014年第12期1564-1565,共2页International Journal of Laboratory Medicine

基　　金：河北省科技厅课题资助项目(20131041)

摘　　要：目的探讨慢性乙型肝炎患者经恩替卡韦治疗24个月后,血清及肝细胞中的乙型肝炎病毒(HBV)共价闭合环状DNA(cccDNA)表达水平的变化情况。方法对126例慢性乙型肝炎患者给予恩替卡韦治疗24个月,采用荧光定量PCR法检测治疗前、后血清中的HBV DNA,血清及肝细胞中的HBV cccDNA水平。结果 126例慢性乙型肝炎患者经恩替卡韦治疗24个月后,有81.75%(103/126)的患者血清中HBV DNA<103 copy/mL。16例慢性乙型肝炎患者在肝细胞中检测到HBV cccDNA,并且其水平在治疗前、后无变化。结论经恩替卡韦治疗的慢性乙型肝炎患者,HBV DNA复制虽然被有效抑制,但肝细胞内的HBV cccDNA并未被彻底清除。Objective To explore the changes of hepatitis B virus （HBV）covalently closed circular DNA （cccDNA）levels in chronic hepatitis B patients after 24 months of treatment with entecavir.Methods Serum levels of HBV cccDNA and HBV DNA, and liver cell levels of HBV cccDNA were detected in 126 patients with chronic hepatitis B before treatment and after treatment of entecavir for 24 months by using quantitative polymerase chain reaction.Results After treatment of entecavir for 24 months,there were 103 patients （81 .75%）were with HBV DNA levels less than 103 copies/mL.HBV cccDNA could be detected in liver cells of 16 patients,but the levels were not with significant difference before and after treatment.Conclusion Although replication of HBV DNA could be effectively suppressed by treatment of entecavir,but HBV cccDNA in liver cells could not be completely removed.

关 键 词：肝炎 乙型 病毒性肝炎 恩替卡韦 共价闭合环状DNA 聚合酶链反应 

分 类 号：R512.62[医药卫生—内科学]