3种HBsAg检测方法的比较  

Comparison of three methods for HBsAg detection

作　　者：王海刚[1] 丁磊[1] 潘航[1] 韩娟[1] 任传路[1] 韩佳[2] 

机构地区：[1]中国人民解放军第一〇〇医院检验科,江苏苏州215007 [2]苏州工业园区蒌葑医院斜塘分院检验科,江苏苏州215009

出　　处：《国际检验医学杂志》2014年第12期1614-1615,共2页International Journal of Laboratory Medicine

摘　　要：目的通过金标法、酶联免疫吸附测定(ELISA)法和光激化学发光(LiCA)法3种方法检测HBsAg,评价3种检测方法在临床上的适用性。方法选取1 000例标本,每一例标本都采用3种方法进行检测,筛查可疑的HBsAg阳性标本进行复检,鉴别假阴性、假阳性。结果 3种检测方法中,LiCA法最为敏感和特异,其次是ELISA法,金标法的灵敏度虽然略低于两者,但金标法最大的特点就是简单、快速,其灵敏度可达1ng/mL,适合急诊或术前患者的初步筛查,能满足临床快速检测的需求。ELISA法由于操作步骤多易造成假阴性、假阳性,但是其灵敏度高、特异性强、价格低廉,更易被大众所接受,适合大批量样本检测。LiCA法虽然价格贵,但其特有的高稳定性、高准确性、易操作性能更好地反映患者体内HBV复制状况以及抗病毒药物的疗效。结论临床应根据需求选择适宜的HBsAg检测方法。Objective To evaluate the clinical applicability of Gold Lebel,ELISA and LiCA,through detecting HBsAg by all the three methods.Methods A total of 1 000 sapmles were selected,and each sample was detected HBsAg by using three methods. Each positive sampale was detected repeatedly to identify false negative and false positive.Results In all the three methods,the most sensitive and specific method was LiCA,next was ELISA,and the last was Gold Lebel.But Gold Lebel method was more sim-ple and rapid for preliminary screening for emergency or preoperative patients with its sensibility of 1 ng/mL.ELISA had too many operating steps to bring false negative and false positive,while its high sensitivity and specificity and low price might be accepted by commonage and fit for mass simples.LiCA′s especially high stability,accuracy,easily operating could better reflex HBV replication and therapy effects of antiviral drugs in spite of its slight cost.Conclusion The suitable HBsAg detection method should be chosen according to clinical demands.

关 键 词：乙肝表面抗原 胶体金免疫层析法 酶联免疫吸附测定 光激化学发光法 

分 类 号：R446.6[医药卫生—诊断学]