HPLC测定灰毡毛忍冬不同部位中绿原酸和木犀草苷的含量  

HPLC Determination of Chlorogenic Acid and Galuteolin in Different Parts of Lonicera macranthoides

作　　者：吴飞燕[1] 卿志星[1] 曾建国[1,2] 

机构地区：[1]湖南中医药大学湖南省植物功能成分利用协同创新中心,湖南长沙410208 [2]湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128

出　　处：《中国现代中药》2014年第8期614-617,共4页Modern Chinese Medicine

基　　金：农业行业标准制定与修订(兽药)(112203);国家科技支撑计划(2012BAI29B04)

摘　　要：目的:利用HPLC测定灰毡毛忍冬不同部位中绿原酸和木犀草苷的含量。方法:采用Agilent ZORBAX SB-phenyl C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.5%醋酸水溶液进行等度洗脱,检测波长为350 nm,流速为1 mL·min-1,柱温为25℃。结果:灰毡毛忍冬不同部位中绿原酸的含量为花>叶>枝;木犀草苷的含量为叶>花>枝。结论:本法简便、灵敏、重复性好,可用于灰毡毛忍冬不同部位样品中绿原酸和木犀草苷的含量测定。Objective： To quantify chlorogenic acid and galuteolin in different parts of Lonicera macranthoides by HPLC method. Methods： The analysis was performed on Agilent ZORBAX SB-phenyl Cls column （250 mm ×4. 6 mm, 5 μm）. The mobile phase composed of acetonitrile-0. 5% acetic acid aq. with isocratic elution. The detecting wavelength was 350 nm. The flow rate was 1 mL. min -1 and the column temperature was 25 ℃. Results： The chlorogenic acid content in different parts of L. macranthoides is flowers 〉 leaves 〉 branches. The galuteolin content is leaves 〉 flowers 〉 branches. Conclusion： The method is simple, sensitive and repeatability, which can be used for determining the content of chlorogenic acid and galuteolin in different parts of L. macranthoides.

关 键 词：灰毡毛忍冬 绿原酸 木犀草苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]