EV71手足口病患儿血清NSE、CK-BB及S-100B检测的临床意义  

Clinical significance of detecting NSE, CK-BB and S-100B in serum on EV71 hand foot and mouth disease

作　　者：王乾[1] 戎小平[2] 王园园[3] 

机构地区：[1]保定市第一医院儿科,河北保定071000 [2]河北医科大学第四医院儿科,石家庄050011 [3]保定市第一中心医院超声科,河北保定071000

出　　处：《中国医学前沿杂志（电子版）》2014年第7期83-85,共3页Chinese Journal of the Frontiers of Medical Science(Electronic Version)

摘　　要：目的探讨EV71手足口病患儿检测神经元特异性烯醇化酶(NSE)、肌酸激酶脑型同工酶(CK-BB)、S-100B蛋白的临床意义。方法选择本院收治的50例重症手足口病患儿为重症组,50例普通型手足口病患儿为普通组,选择30例健康儿童为对照组,采用酶联免疫吸附法测定三组研究对象血清NSE和S-100B蛋白表达水平。采用电泳法检测CK-BB表达水平,比较三组研究对象血清NSE、S-100B及CK-BB表达水平的差异。结果重症组、普通组、对照组血清NSE、CK-BB、S-100B表达水平存在显著差异(P<0.01),重症组患儿血清NSE、CK-BB、S-100B表达水平高于普通组,重症组及普通组患儿血清NSE、CK-BB、S-100B表达水平均高于对照组。结论手足口病患儿血清NSE、CK-BB和S-100B表达水平升高,其血清浓度与病情相关,检测血清NSE、CK-BB和S-100B表达水平对手足口病患儿的早期诊断及预后评估具有参考意义。Objective To investigate the linical significance of detecting NSE, CK-BB and S-100 B in serum on children with EV71 hand foot and mouth disease. Method 50 cases of children with severe hand foot and mouth disease were selected as severe group, 50 cases of children with common hand foot and mouth disease were selected as common group, and 30 cases of normal children were selcected as control group, concentration of NSE and S-100 B proteinum in serum were detected with enzyme linked immunosorbent assay, concentration of CK-BB were detected with electrophoretic method. and level of NSE, S-100 B and CK-BB were contrasted along three groups. Result Levels of NSE, CK-BB and S-100 B in serum were notable different along three groups（P〈0.05）, levels of NSE, CK-BB and S-100 B were higher in severe group and common group than in control groups（P〈0.05）, levels of NSE, CK-BB and S-100 B were higher in severe group than in common group（P〈0.05）. Conclusion Levels of NSE, CK-BB and S-100 B in serum increasing in children with EV71 hand foot and mouth disease, the and concentration is correlative with pathogenetic condition, and detecting NSE, CK-BB and S-100 B in serum is significative for early diagnosis and evaluating prognosis.

关 键 词：EV71手足口病 神经元特异性烯醇化酶 肌酸激酶脑型同工酶 S-100B蛋白 

分 类 号：R725.1[医药卫生—儿科]