猪脂肪诱导转录物的克隆与组织分布  

Molecular cloning and tissue distribution of porcine fat-induced transcript

作　　者：王静[1] 李巍[1] 

机构地区：[1]河南牧业经济学院,河南郑州450011

出　　处：《郑州牧业工程高等专科学校学报》2014年第2期25-27,共3页Journal of Zhengzhou College of Animal Husbandry Engineering

摘　　要：本试验研究了猪脂肪代谢的重要功能基因——脂肪诱导转录物（FIT）.根据Genebank提供的电子延伸序列和内标β-actin基因序列设计引物,分别以脂肪和肌肉组织mRNA为模板,经反转录和PCR反应,克隆得到了FIT1基因和FIT1 mRNA3＇ UTR区.对测序结果进行序列分析表明,猪FIT1基因的cDNA序列,全长为1 310 bp,ORF为400～1 272 bp,编码290个氨基酸.同源分析结果表明,猪FIT1基因与Genebank上已登录的牛、人、大鼠和小鼠的核酸序列同源性分别为93.2％、92.1％、87.4％和88.1％,氨基酸序列的同源性分别为96.6％、97.2％、94.8％和95.5％.在此基础上,进一步研究了组织分布,显示结果为在肌肉和脂肪组织FIT1有明显分布,而在其它组织分布量很低.The experimental study of the important functions of pig fat metabolism gene -- fat--induced transcript （FIT）. According Genebank provides an electronic extension of the sequence and the internal standard β--actin gene primers were designed, respectively, fat and muscle tissue mRNA as a template, reverse transcription and PCR reactions, the FIT1 gene was cloned and FIT1 mRNA3＇UTR area. Sequence analysis of the sequencing results showed that, cDNA sequence of the porcine gene FIT1 length is 1310bp, ORF of 400--1272bp, encoding 290 amino acids. Homology analysis showed that porcine FIT1 and nucleic acid sequences of bovine, human, rat and mouse were 93. 2% .92.1% .87.4% and 88.1% and amino acid sequence homology was 96.6%.97.2% .94.8% and 95.5%. Based on this, further studies of tissue distribution, the result is displayed in the muscle and adipose tissue distribution FIT1 significantly, and in other low tissue distribution.

关 键 词：猪 脂肪诱导转录物 电子克隆 同源分析 组织分布 

分 类 号：S828[农业科学—畜牧学]