Identification and rDNA-ITS Sequence Analysis of a New Anthracnose Pathogen of Dracaena fragrans in Yunnan Province  

云南巴西木新炭疽病病原菌的鉴定及rDNA-ITS序列分析（英文）

作　　者：吴丽芳[1] 杨海艳[2,3] 魏晓梅[1] 

机构地区：[1]曲靖师范学院生物资源与环境科学学院,云南曲靖655011 [2]楚雄师范学院,云南楚雄675000 [3]云南省高校滇中民族植物学重点实验室,云南楚雄675000

出　　处：《Agricultural Science & Technology》2014年第8期1274-1276,1287,共4页农业科学与技术（英文版）

摘　　要：In this study, identification and rDNA-ITS sequence analysis of an anthracnose pathogen on Dracaena fragrans were carried out. [Method] D. fra-grans leaves with lesions were used as experimental materials to isolate anthrac-nose pathogen. Morphological observation, rDNA-ITS amplification and sequence analysis were performed to identify the pathogen strain. [Result] Caonidia of the iso-lated anthracnose pathogen were straight or curved, el iptic to crescent, with 2-5 oil droplets, 7.5-20 × 4.5-5 μm. According to molecular phylogenetic analysis, the iso-lated pathogen strain was identified as a new species, which was named Col-letotrichum dracaena-fragrantis sp. nov. [Conclusion] This study provided theoretical basis for the prevention and control of anthracnose.[目的]对巴西木炭疽病病原菌进行鉴定及rDNA-ITS序列分析。[方法]该文对巴西木炭疽病病原菌进行分离、形态学观察。通过rDNA-ITS的扩增与序列测定,对rDNA-ITS进行序列分析。[结果]该菌分生孢子直或弯,椭圆形至新月形,2-5个油滴,7.5-20×4.5-5μm。经ITS系统学分析,确定该试验分离到的刺盘孢为一新种,并命名为Colletotrichum dracaena-fragrantis sp.novA。[结论]为炭疽病防止研究提供理论依据。

关 键 词：Dracaena fragrans Col etotrichum ANTHRACNOSE ITS sequence analysis 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]