HPLC法测定复方樟脑软膏中樟脑的含量  

Content determination of camphor in compound camphor ointment by HPLC

作　　者：白林[1] 范金钊[2] 徐风华[1] 王欢欢[1] 

机构地区：[1]解放军总医院药品保障中心,北京100853 [2]蚌埠医学院,安徽蚌埠233000

出　　处：《中国药物应用与监测》2014年第4期212-213,258,共3页Chinese Journal of Drug Application and Monitoring

基　　金：半固体制剂标准提高系列研究课题(13ZJZ05)

摘　　要：目的：建立HPLC法测定复方樟脑软膏中樟脑的含量。方法：采用色谱柱Diamonsil C18柱（250 mm×4.6 mm,5μm）;流动相：甲醇-乙腈-水（15∶45∶40）;检测波长：289 nm;柱温：35℃;流速：1.0 mL·min-1;进样量：10μL。结果：樟脑与其他杂质峰分离良好,樟脑在250.00-2 000.00μg·mL-1范围内与峰面积呈良好的线性关系（r=1.000 0）,平均回收率为99.68%,RSD为1.21%（n=9）。结论：本方法简便易行、准确,可作为复方樟脑软膏中樟脑的质量控制方法。Objective： To establish the HPLC method for determining the content of camphor in compound camphor ointment. Methods：The analysis was carried out on a column of Diamonsil C18 （250 mm ＆#215; 4.6 mm, 5μm） with the mobile phase consisting of methanol-acetonitrile-water （15∶45∶40）, the flow rate was 1.0 mL＆#183;min-1, the detection wavelength was 289 nm, column temperature was 35℃, the sample injection was 10μL. Results：The samples were separated well from other substances. The calibration curve of camphor was linear in the range of 250.00-2 000.00 μg·mL^-1 （r = 1.000 0）. The average recovery of camphor in the compound camphor ointment was 99.68%with RSD of 1.21%（n=9）. Conclusion： This method is accurate and feasible, which can be applied as the quality control of camphor in the compound camphor ointment.

关 键 词：高效液相色谱法 复方樟脑软膏 樟脑 含量测定 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]