miRNA-26a在良、恶性腹水鉴别诊断中的意义  

Value of miRNA-26a level in benign or malignant ascites

作　　者：李曦[1] 何敏[1] 邹兵[1] 

机构地区：[1]北京大学深圳医院消化内科,广东深圳518036

出　　处：《中国医药导报》2014年第23期51-53,75,共4页China Medical Herald

基　　金：广东省深圳市科技计划项目(编号201203003)

摘　　要：目的探讨miRNA-26a在良、恶性腹水鉴别诊断中的意义。方法收集北京大学深圳医院2011年9月～2014年3月门诊及住院患者60例,包括20例肝硬化腹水患者(肝硬化腹水组),20例结核性腹水患者(结核性腹水组),20例恶性腹水患者(恶性腹水组)。采用实时定量PCR(qRT-PCR)方法检测并比较各组血清miRNA-26a、甲胎蛋白(AFP)、糖类抗原125(CA125)的表达。结果恶性腹水组血清CA125表达高于结核性腹水组和肝硬化腹水组,但差异无统计学意义(P>0.05)。AFP、miRNA-26a在结核性腹水组及肝硬化腹水组表达差异无统计学意义(P>0.05);AFP和miRNA-26a在恶性腹水组患者血清表达明显高于结核性腹水组和肝硬化腹水组,差异有统计学意义(P<0.05)。结论 miRNA-26a水平将来有可能有助于良、恶性腹水的鉴别诊断。Objective To investigate the value of serum miRNA-26a level in patients with benign or malignant ascites. Methods 60 cases in Peking University Shenzhen Hospital from September 2011 to March 2014 were included and di-vided into hepatic cirrhosis ascites group with 20 cases, tuberculous ascites group with 20 cases and malignant ascites group with 20 cases. miRNA-26a, AFP, CA125 level in blood serum were measured by qRT-PCR. Results The serum CA125 in malignant ascites group was higher than those in hepatic cirrhosis ascites group and tuberculous ascites group, but the difference was not statistically significant （P〉0.05）. The differences of AFP, miRNA-26a between tuber-culous ascites group and hepatic cirrhosis ascites group was not statistically significant （P〉0.05）;the AFP, miRNA-26a in malignant ascites group were all higher than those in hepatic cirrhosis ascites group and tuberculous ascites group, the differences were statistically significant （P〈 0.05）. Conclusion Diagnosis of benign or malignant ascites may bene-fit from the measurement of miRNA-26a level.

关 键 词：miRNA-26a 肝硬化腹水 结核性腹水 恶性腹水 

分 类 号：R442.5[医药卫生—诊断学]