成年男性阴囊局部热压后精液一氧化氮及精子DNA改变  

Study on changes in seminal NO, sperm parameters and sperm DNA integrity by using 43 ℃ scrotal heat stress for 23 adult men

作　　者：仕治达 张美华 王磊光 陈峰 刘华强 邱毅 

机构地区：[1]山东省计划生育科学技术研究所山东省优生技术重点实验室,济南250002 [2]山东省新泰市计划生育服务站 [3]山东省平阴县计划生育服务站

出　　处：《中华临床医师杂志（电子版）》2014年第15期32-36,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：山东省重大科技专项(SDSP2005-0410-06-2005GG1108001);"十二五"国家科技支撑计划(2012BAI31B08)

摘　　要：目的观察成年生育男性阴囊在局部热压后血清生殖激素水平、精液参数、一氧化氮(NO)及精子DNA完整性的改变。方法 23例已育成年健康男性志愿者阴囊外部放置恒温加热带(阴囊热压,SHS),热压温度43℃,每周2次,每次50 min,连续3个月。化学发光免疫分析法测定血清生殖内分泌激素T、FSH、LH,硝酸还原法测定精液NO含量,精液常规分析、精子低渗肿胀试验(HOS)及精子染色质扩散试验(SCD)分别在试验开始前和试验后进行。SHS试验前后进行自身对照。结果 SHS试验1、2及3个月血清T水平、精子密度及存活率比试验前明显下降(P<0.01),FSH及LH水平、畸形精子、精液NO含量及精子DNA碎片明显增高(P<0.001)。停止SHS试验3个月后上述各项指标又恢复到试验前水平。精子存活率与精子畸形率呈正相关(r=0.496,P=0.016),精液NO含量与精子密度呈负相关(r=-0.497,P=0.016),与畸形精子比率及精子DNA碎片比率呈正相关(r=0.510,P=0.013;r=0.508,P=0.014)。结论短期恒温睾丸热压能够影响精液质量,抗生育效果明显,SHS停止后可较快恢复。Objective To observe changes in reproductive hormone, semen parameters, seminal nitric oxide （NO） and sperm DNA of adult healthy men after scrotal heat stress （SHS）. Methods Exposure of the scrotum of 23 healthy male volunteers （have children） locally at 43℃belt warming 50 min each day for successive 2 d per week. The course of SHS was continuously 3 months. The reproductive hormone level of follicle stimulating hormone （FSH）, luteinizing hormone （LH） and testosterone （T） in serum was measured by chemiluminescent immunoassay. Routine semen analysis, hypo-osmotic swelling （HOS） test and determination of sperm DNA fragmentation by sperm chromatin dispersion （SCD） test were carried on before, during and after SHS. Seminal NO content was determined by nitrate reduction method. Self-control study was used. Results The mean parameter of sperm density, vitality, and serum T level was significantly decreased, in contrast, the mean abnormal sperm, sperm DNA fragmentation, seminal NO level, and serum FSH and LH was significantly increased for sperm collected during SHS 1, 2 and 3 months vs. pre-SHS （P〈0.01-0.001）. The SHS was stopped for 3 months, the various parameters restored to the level of before SHS. The significant positive correlation was found between sperm vitality and abnormal sperm （r=0.496, P=0.016）, and between seminal NO level and the mean abnormal sperm, sperm DNA fragmentation （r=0.510, P=0.013 and r=0.508, P=0.014）. The significant negative correlation was found between sperm density and seminal NO level （r=-0.497, P=0.016）. Conclusion The continuously constant SHS can impact the semen quality and serum reproductive hormone of adult men, and the anti-fertility of SHS is excellent.

关 键 词：精子发生 一氧化氮 DNA碎片裂 避孕 睾丸热压 

分 类 号：R698.2[医药卫生—泌尿科学]