ICU患者多重耐药定植菌与感染菌基因同源性研究  

Study on gene homology of multi-drug resistant colonized bacteria and infectious bacteria in ICU patients

作　　者：常洪美[1] 凌冬[2] 张丕[3] 陈开全[1] 戴忠红[3] 柴建华[1] 

机构地区：[1]崇州市人民医院院感科,四川崇州611230 [2]崇州市人民医院检验科,四川崇州611230 [3]崇州市人民医院ICU,四川崇州611230

出　　处：《国际检验医学杂志》2014年第16期2137-2138,共2页International Journal of Laboratory Medicine

基　　金：四川省成都市科技局基金资助项目(12PPYB092SF-002)

摘　　要：目的研究ICU患者肠道定植菌与感染菌基因的同源性,为制定控制多重耐菌医院感染措施提供流行病学和分子生物学依据。方法对ICU患者肛拭子分离出的多重耐药定植菌与同一患者临床标本分离出的相同多重耐药菌进行配对,采用Diversilab自动化重复序列-PCR分型系统,分析肠道多重耐药定植菌与感染菌基因的同源性。结果选取4对多重耐药定植菌与患者入院时临床标本分离出的相同多重耐药菌进行同源性检测,2对有同源性、2对无同源性;选取4对多重耐药定植菌与患者医院感染临床标本分离出的相同多重耐药菌进行同源性检测,4对均有同源性。结论 ICU患者多重耐药定植菌与感染菌基因具有同源性,多重耐药定植菌可以引起医院感染的发生,临床上应加强多重耐药定植菌患者的管理。Objective To study the gene homology of intestinal colonized and infectious bacteria in ICU patients to provide the epidemiological and molecular biological basis for formulating the control measures of multi resistant bacterial hospital infection. Methods The multi-drug resistant colonized bacteria isolated from the anal swabs and the same multi-drug resistant bacteria isola-ted from the clinical samples in the same patients were matched.The Diversilab automatic repetitive extragenic palindromic（REP）-PCR typing system was adopted to analyze the gene homology of multi-drug resistant colonized bacteria and infectious bacteria in the intestinal tract.Results 4 pairs of multi-drug resistant colonized bacteria and the same multi-drug resistant bacteria isolated from the clinical samples on admission in the same patients were selected and performed the homology detection,2 pairs had the ho-mology and 2 pairs had no homology;4 pairs of multi-drug resistant colonized bacteria and the same multi-drug resistant bacteria isolated from the clinical samples in the patients with hospital infection were performed the homology detection,4 pairs all showed the homology.Conclusion The multi-drug resistant colonized bacteria and the infectious bacteria in ICU patients have the homolo-gy.The multi-drug resistant colonized bacteria can cause the occurrence of hospital infection,so their management should be strengthened in clinic.

关 键 词：多重耐药菌 医院感染 基因 

分 类 号：R440[医药卫生—诊断学]