医院门诊部与住院部人员nuc-mecA基因携带调查分析  

Investigation and analysis on carrying nuc-mecA gene situation in outpatient and inpatient departments

作　　者：胡娟[1] 薛怀裕[1] 呼永河[2] 

机构地区：[1]成都军区临床医学检验中心,四川成都610083 [2]成都军区总医院中医科,四川成都610083

出　　处：《国际检验医学杂志》2014年第16期2189-2191,共3页International Journal of Laboratory Medicine

基　　金：美国医学研究基金(亚洲区)临床微生物学研究基金(CNSC-J2011-A330)

摘　　要：目的：调查医院住院部和门诊部人员携带 nuc-mec A 基因的情况，为院感控制提供数据，达到防止院内感染、提高治愈率的目的。方法将调查对象分为4组：骨关节及偏瘫康复科组（A 组）44例、综合外科及血液肿瘤科组（B 组）32例、心肺科组（C 组）17例、门诊组（D 组）35例，A、B、C 组为住院部人员，D 组为门诊部人员，年龄为30～84岁。收集其鼻拭子样本，采用多通道实时荧光 PCR 技术检测 nuc-mec A 基因情况，探讨不同部门、不同人群 nuc-mec A 基因携带情况。结果 A、B、C 组 nuc 基因携带率为0．00％，D 组 nuc 基因携带例数为1例，携带率为2．86％，4组比较差异无统计学意义（P ＞0．05）；A、B、C、D 组 mecA 基因携带例数为26例、17例、13例、10例，携带率分别为59．10％、53．10％、76．50％、28．60％，差异有统计学意义（P ＜0．01），但 A、B、C 组 mecA 基因携带率差异无统计学意义（P ＞0．05）；医院整体患者和医护人员 nuc-mec A 基因携带率差异有统计学意义（P ＜0．05），但各组的患者和医护人员 nuc-mec A 基因携带率差异无统计学意义（P ＞0．05）。结论 nuc-mec A 基因普遍存在于住院部中，门诊部 nuc-mec A 基因携带率相对较少。提示住院部要注意病原菌的预防和控制，特别是对偏瘫康复科和心肺科的去定植，防止不同科室之间以及与门诊部的交叉感染。Objective To investigate the carrying situation of nuc-mec A gene for different departments in the inpatient depart-ment and outpatient department to provide the data for the hospital infection control and reaching the target for preventing nosoco-mial infection and improving the cure rate.Methods The research subjects were divided into 4 groups：osteoarthropathy and hemi-plegia rehabilitation department group（A,44 cases）,comprehensive surgical and hematology-oncology department group（B,32 ca-ses）,cardiopulmonary department group（C,17 cases）and outpatient department group（D,35 cases）.The group A,B and C were the persons in the inpatient department and the group D were the persons in the outpatient department,aged 30-84 years old.Na-sal swab samples were collected and the nuc-mec A gene was detected by the multi-channel real-time PCR.Results No person in the group A,B and C carried nuc gene and 1 case（2.86%）in the group D carried nuc gene.There were no statistically significant differ-ence among 4 groups（P〉0.05）;the carriers of mecA gene in the group A,B,C and D were 26,17,13 and 10 cases,the carrying rates were 59.10%,53.10%,76.50% and 28.60% respectively,the difference showed statistical significance（P 〈0.01）,but which in the group A,B and C had no statistically significant difference（P〉0.05）;the carrying rate of nuc-mec A gene showed statistically significant differences between the patients and the medical staff（P〈0.05）,but which had no statistically significant differences be-tween the patients and the medical staff in each group（P 〉0.05）.Conclusion nuc-mec A gene generally exists in the inpatient de-partment and the carrying rate of nuc-mec A gene is relatively lower,which indicates that the inpatient department needs to pay at-tention to the prevention and treatment of pathogenic bacteria,especially the de-clonization in the hemiplegia rehabilitation and car-diopulmonary departments for avoiding cross-infection among different departments.

关 键 词：基因 住院部 门诊部 

分 类 号：R440[医药卫生—诊断学]