MALDI-TOF质谱仪在临床常见细菌鉴定中的应用  

Application of matrix-assisted laser desorption ionization-time of flight mass spectrometer in identification of clinical common bacteria

作　　者：王冰[1] 任晓庆[1] 褚美玲[1] 杨柳[1] 盛翔宇 周连庆[1] 薛文成[1] 孟冬娅[1] 

机构地区：[1]沈阳军区总医院检验科,辽宁沈阳110016

出　　处：《国际检验医学杂志》2014年第16期2228-2230,共3页International Journal of Laboratory Medicine

基　　金：辽宁省科技攻关计划(2011225021)

摘　　要：目的探讨两种质谱仪Biotyper MS和Vitek-MS系统在临床常规分离细菌鉴定中的应用价值。方法收集沈阳军区总医院2012年3月至2013年1月分离自血液、尿液、脑脊液、分泌物和痰液等标本的临床常见菌细菌149株(包括14个菌属和30个菌种)。同时采用两种MALDI-TOF-MS系统对上述菌株进行鉴定,结果与Vitek2compact常规生化鉴定进行比较,对3种方法检测结果不一致菌株用16Sr RNA测序进行确认。结果在149株常见细菌中,Bruker Biotype属和种的正确鉴定率分别为98%和96%,Vitek-MS属和种的正确鉴定率分别为97%和95%。两者均无错误鉴定菌株。结论对该组常见细菌,两种质谱仪鉴定结果比较差异无统计学意义(P>0.05),均呈现出优异的鉴定水平,适合用于临床微生物的常规鉴定。Objective To evaluated the application value of two kinds of mass spectrometer（MS）and Vitek MS system in the i-dentification of routinely isolated bacteria in clinic.Methods 149 strains of common bacteria（including 14 genera and 30 species）i-solated from blood,urine,cerebral spinal fluid,secretion and sputum samples in our hospital from March 2012 to January 2013 were collected and simultaneously identified by 2 kinds of matrix-assisted laser desorption ionization-time of flight mass spectrometer （MALD-TOF-MS）.The identification results were compared with those identified by the conventional biochemical identification （Vitek2 compact）.The strains with the inconsistent results identified by 3 kinds of method were confirmed by 16S rDNA gene se-quencing.Results Among 149 common bacteria,the correct identification rates of genus and species by the Bruker Biotyper MS were 98% and 96% respectively and which by the Vitek MS system were 97% and 95% respectively.There were no misidentified bacterial strains by these two kinds of MS.Conclusion No statistical difference in the identification results was observed between these two kinds of MS system（P〉0.05）.Both exhibit excellent identification level and are suitable for the routine laboratory iden-tification of clinical microorganism.

关 键 词：细菌 质谱分析 

分 类 号：R446.5[医药卫生—诊断学]